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Phytochemical and Antibacterial Potential of Vernonia amygdalina Leaf Extracts Against Two Bacteria


Authors : Annu Choudhary; Akanksha Langiwar; Randhir Kumar

Volume/Issue : Volume 11 - 2026, Issue 8 - August


Google Scholar : https://tinyurl.com/45c68k6x

DOI : https://doi.org/10.38124/ijisrt/26aug1019

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : The study aims to evaluate the phytochemical screening and antibacterial activity of Vernonia amygdalina leaves extracts against microorganism Staphylococcus aureus and Escherichia coli. Phytochemical study revealed that numerous bioactive components were found in the cold and hot water and ethanol extracts of the plant. Ethanol and water extractions were used to analyze the effects of V. amygdalina leaf extracts, and the well diffusion method was used to assess antibacterial activity.

Keywords : Vernonia amygdalina, Aqueous Extract, Antimicrobial Resistances, Ethanol Extract, Zone of Inhibition, Well Diffusion Method.

References :

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  2. Anibijuwon, I. I., Oladejo, B. O., Adetitun, D. O., Kolawole, O. M., Public Health Laboratory Unit, Department of Microbiology, School of Science. (2012). Antimicrobial Activities of Vernonia amygdalina Against Oral Microbes (journal-article). (IDOSI Publications), Global Journal of Pharmacology (Vol. 6, pp. 178–185). https://doi.org/10.5829/idosi.gjp.2012.6.3.6517
  3. Bello, A., Galadima, M. S., & Aliyu, F. S. (2024). Comparative study of phytochemicals composition of Mangifera indica (mango) and Vernonia amygdalina (bitter leaf) leaves and their antimicrobial activities. International Journal of Scientific Research in Chemical Sciences Vol, 11(6).
  4. Choudhary, A., Langiwar, A., & Kumar, R. (2026). Study on Morphology, Anatomy, Phytochemical and Antimicrobial Activity of Terminalia catappa L. Leaves. International Journal of Current Microbiology and Applied Sciences, 15(4), 8–19. https://doi.org/10.20546/ijcmas.2026.1504.002
  5. Galadima, A. M., Chidimma, A. J., Abubakar, I., Ibrahim, S., & Said, S. S. (2025). Phytochemicals Composition and Anti-bacterial Activity of Methanol Leaves Extract of Vernonia amygdalina. Biology Medicine & Natural Product Chemistry, 14(1), 519–527. https://doi.org/10.14421/biomedich.2025.141.519-527
  6. Jarmai, A. H., Sheikh, A. M., Onyiche, T. E., Yunus, H., Aji, M. A., & Umar, S. M. (2022). Antimicrobial Activity and Phytochemical Screening of Methanolic Leaf Extract of Vernonia amygdalina. South Asian Journal of Research in Microbiology, 23–35. https://doi.org/10.9734/sajrm/2022/v14i1263
  7. Jin, Z., Gao, L., Zhang, L., Liu, T., Yu, F., Zhang, Z. & Wang, B. (2017). Antimicrobial activity of saponins produced by two novel endophytic fungi from Panax Noto ginseng. Natural Product Research, 31: 2700–2703.https://doi.org/10.1080/14786419.2017.1292265
  8. Magaji, A., Mahmud, Z., & Mustafa, A. (2023). Phytochemical Analysis and Assessment of Antibacterial Efficacy of Vernonia amygdalina (Bitter Leaf) against Some Selected Clinical Bacterial Isolates. UMYU Journal of Microbiology Research (UJMR), 8(2), 174–180. https://doi.org/10.47430/ujmr.2382.020
  9. Ofodile, S. N. (2025). ASSESSING THE PHYTOCHEMICAL CONTENTS AND ANTIMICROBIAL ACTIVITY OF BITTER LEAF (VERNONIA AMYGDALINA) ON MICRO-ORGANISMS. International Journal of Functional Research in Science and Engineering (IJFRSE), 3(1).
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The study aims to evaluate the phytochemical screening and antibacterial activity of Vernonia amygdalina leaves extracts against microorganism Staphylococcus aureus and Escherichia coli. Phytochemical study revealed that numerous bioactive components were found in the cold and hot water and ethanol extracts of the plant. Ethanol and water extractions were used to analyze the effects of V. amygdalina leaf extracts, and the well diffusion method was used to assess antibacterial activity.

Keywords : Vernonia amygdalina, Aqueous Extract, Antimicrobial Resistances, Ethanol Extract, Zone of Inhibition, Well Diffusion Method.

Paper Submission Last Date
31 - October - 2026

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