Authors : Khushabu Naresh Magar; Dr. Shahzeen Mohammad Khan

Volume/Issue : Volume 11 - 2026, Issue 3 - March

Google Scholar : https://tinyurl.com/37x3y6ws

Scribd : https://tinyurl.com/3dtvymbj

DOI : https://doi.org/10.38124/ijisrt/26mar306

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Abstract : Sphagneticola trilobata (Asteraceae) is a widely used medicinal plant known for its antimicrobial and antiinflammatory properties. This study aimed to perform a detailed preliminary phytochemical screening and histochemical localization of active compounds in the leaves of S. trilobata. Shade-dried leaves were subjected to sequential extraction using solvents of varying polarity (Petroleum ether, Chloroform, Methanol, and Water). Qualitative phytochemical analysis revealed the presence of alkaloids, flavonoids, terpenoids, tannins, and glycosides, with methanol extract showing the highest diversity of metabolites. Histochemical investigations of leaf tissues using Sudan III and Nadi reagent confirmed the localization of lipids and terpenoids within the glandular trichomes and internal secretory canals. These findings provide baseline data for the standardization, quality control, and further isolation of active compounds from S. trilobata.

References :

1. Ekpo M., Mbagwu H., Jackson C. and Eno M., Antimicrobial and wound healing activities of Centrosema pubescens (Leguminosae), J. Phys. Chem. Solids, 2011; 1: 1-6.
2. Bazzaz B.S.F., Arab A., Emami S.A., Asili J., Hasanzadeh-Khayyat M. and Sahebkar A., Antimicro- bial and antioxidant activities of methanol, dichloro- methane and ethyl acetate extracts of Scutellaria lindbergii Rech.f., Chiang Mai J. Sci. 2013; 40: 49-59.
3. Pradhan D., Panda P.K. and Tripathy G., Wound healing activity of aqueous and methanolic bark extracts of Vernonia arborea Buch-Ham. in Wistar rats, Nat. Prod. Rad., 2009; 8: 6-11.
4. Joseph B.S. and Justin R.S., A comparative study on various properties of five medicinally important plants, Int. J. Pharmacol., 2011; 7: 206-211.
5. Clare B.A., Conroy R.S. and Spelman K., The diuretic effect in human subjects of an extract of Taraxacum officinale folium over a single day, J. Altern.
6. Complement. Med., 2009; 15: 929-934.6. Harbone SV. 6) Biochemistry of plant Phenolies, Recent Advances in Phytochemistry. 1973; 12. 760.
7. Harborne JB. 'Phytochemical Methods'. A guide to modern techniques of plant analysis 3rd Edn. Chapman and Hall, New York. 1998; 1-150.
8. Kumudini IT, Shubha G, Deepak K, Agrwal S, Anil Kumar. Antimicrobial activity of Sphagneticola trilobata (L.) Pruski, against some human pathogenic bacteria and Fungi. The Bioscan. 2013; 8(2):695-700.
9. Li D, Liang Z, Guo M, Zhou J, Yang X, Xu J. Study on the chemical composition and extraction technology optimization of essential oil from Wedelia trilobata (L.) Hitchc. African Journal of Biotechnology. 2012; 11(20):4513-4517.