Phytochemical Profiling of the Aqueous Methanolic Extract of the Traditional Polyherbal Product Batrisu Vasanu Using GC – MS


Authors : Sanket Charola; Yashvi Adhiya; Anjali Dabhi; Maya Choudhary

Volume/Issue : Volume 10 - 2025, Issue 8 - August


Google Scholar : https://tinyurl.com/mtze7by4

Scribd : https://tinyurl.com/mr24sb6k

DOI : https://doi.org/10.38124/ijisrt/25aug785

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Note : Google Scholar may take 30 to 40 days to display the article.


Abstract : Batrisu vasanu is an ethnobotanically practiced nutraceutical believed to increase lactation and benefit new mothers in postpartum recovery. This mixture of 32 herbs is polyherbal in nature and requires the phytochemical profiling to better understand its clinical effect. With an aim to profile the phytochemicals, GC-MS was performed with aqueous methanolic extract of this polyherbal mixture. The sample showed various bioactive compounds present in the mixture. The GC-MS analysis revealed the presence of twenty-two phytochemicals namely Trifluoromethyl t-butyl disulfide, L-(+)-Ascorbic acid 2,6-dihexadecanoate, Bromoacetic acid, 2-ethylhexyl ester, and Farnesol isomer-A. Peak 5 at retention time 19.741 showed highest percentage area under the peak that was 22.742. The present study with phytochemical profiling of Batrisu vasanu revealed the presence of numerous bioactive compounds with effective medicinal properties. It can be concluded that the medicinal effect of Batrisu vasanu is due to these key bioactive phytochemicals.

Keywords : Batrisu Vasanu; Galactagogue; Phytochemicals; GC-MS; Polyherbal.

References :

  1. Charola, S., Tadvi, B., Albert, S., Suresh, B., & Nair, S. (2021). Analysis of a polyherbal galactagogue Batrisu vasanu, an indigenous Indian ethnomedicine. Indian Journal of Natural Products and Resources, 12(4), 610–616.
  2. Ghasemi, V., Kheirkhah, M., & Vahedi, M. (2015). The Effect of Herbal Tea Containing Fenugreek Seed on the Signs of Breast Milk Sufficiency in Iranian Girl Infants. Iranian Red Crescent Medical Journal, 17(8), e21848.
  3. Kumar, S., Sandhir, R., Ojha, S., Gautam, S., Gautam, A., Chhetri, S., Bhattarai, U., Duda-Chodak, A., Tarko, T., Rus, M., Adedapo, A. A., Jimoh, F. O., Afolayan, A. J., Masika, P. J., Momoh, J. O., Manuwa, A. A., Oshin, T. T., Pratap, R., Aswatha Ram, H. N., … Zimare, S. B. (2011). Antioxidant activities and phenolic contents of the methanol extracts of the stems of Acokanthera oppositifolia and Adenia gummifera. BMC Complementary and Alternative Medicine, 11(3), 485–492.
  4. Malik, Z. A., Bhat, J. A., Ballabha, R., Bussmann, R. W., & Bhatt, A. B. (2015). Ethnomedicinal plants traditionally used in health care practices by inhabitants of Western Himalaya. Journal of Ethnopharmacology, 172, 133–144.
  5. Michel, J. L., Caceres, A., & Mahady, G. B. (2016). Ethnomedical research and review of Q’eqchi Maya women’s reproductive health in the Lake Izabal region of Guatemala: Past, present and future prospects. Journal of Ethnopharmacology, 178, 307–322.
  6. Morvin Yabesh, J. E., Prabhu, S., & Vijayakumar, S. (2014). An ethnobotanical study of medicinal plants used by traditional healers in silent valley of Kerala, India. Journal of Ethnopharmacology, 154(3), 774–789.
  7. Nordeng, H., & Havnen, G. C. (2004). Use of herbal drugs in pregnancy: A survey among 400 Norwegian women. Pharmacoepidemiology and Drug Safety, 13(6), 371–380.
  8. Olivia, N. U., Goodness, U. C., & Obinna, O. M. (2021). Phytochemical profiling and GC-MS analysis of aqueous methanol fraction of Hibiscus asper leaves. Future Journal of Pharmaceutical Sciences 2021 7:1, 7(1), 1–5.
  9. Pan, M. H., Chiou, Y. S., Tsai, M. L., & Ho, C. T. (2011). Anti-inflammatory activity of traditional chinese medicinal herbs. Journal of Traditional and Complementary Medicine, 1(1), 8–24.
  10. Sanket, C., Patlavath, R., Desai, M., Lency, P., Pujan, K., Afsha, A., & Charmi, P. (2025). A Comparative Qualitative Phytochemical Analysis of in-House and Commercial Polyherbal Formulations Using Thin Layer Chromatography. Indian Journal of Plant Sciences, 14, 1–5.
  11. Thangliankhup, K., Lalfakawma, Gouda, S., & Khomdram, S. D. (2022). Ethnomedicinal plants of Kuki-Chin tribes in Kaihlam wildlife sanctuary of Manipur, India. Acta Ecologica Sinica.
  12. Watcho, P., Ngadjui, E., Alango Nkeng-Efouet, P., Benot Nguelefack, T., Kamanyi, A., Tabares, F. P., Jaramillo, J. V. B., Ruiz-cortés, Z. T., WHO, Barennes, H., Simmala, C., Odermatt, P., Thaybouavone, T., Vallee, J., Martinez-Ussel, B., Newton, P. N., Strobel, M., Yeon, J., Yun, J., … E., M. (2019). The use of medicinal herbs in gynecological and pregnancy-related disorders by Jordanian women: a review of folkloric practice vs. evidence-based pharmacology. Journal of Ethnopharmacology, 15(1), 100224.
  13. Yarazari, S. B., & Jayaraj, M. (2022). GC–MS Analysis of Bioactive Compounds of Flower Extracts of Calycopteris floribunda Lam.: A Multi Potent Medicinal Plant. Applied Biochemistry and Biotechnology, 194(11), 5083–5099.
  14. Zuppa, A. A., Sindico, P., Orchi, C., Carducci, C., Cardiello, V., Romagnoli, C., & Catenazzi, P. (2010). Safety and efficacy of galactogogues: Substances that induce, maintain and increase breast milk production. In Journal of Pharmacy and Pharmaceutical Sciences (Vol. 13, Issue 2, pp. 162–174).

Batrisu vasanu is an ethnobotanically practiced nutraceutical believed to increase lactation and benefit new mothers in postpartum recovery. This mixture of 32 herbs is polyherbal in nature and requires the phytochemical profiling to better understand its clinical effect. With an aim to profile the phytochemicals, GC-MS was performed with aqueous methanolic extract of this polyherbal mixture. The sample showed various bioactive compounds present in the mixture. The GC-MS analysis revealed the presence of twenty-two phytochemicals namely Trifluoromethyl t-butyl disulfide, L-(+)-Ascorbic acid 2,6-dihexadecanoate, Bromoacetic acid, 2-ethylhexyl ester, and Farnesol isomer-A. Peak 5 at retention time 19.741 showed highest percentage area under the peak that was 22.742. The present study with phytochemical profiling of Batrisu vasanu revealed the presence of numerous bioactive compounds with effective medicinal properties. It can be concluded that the medicinal effect of Batrisu vasanu is due to these key bioactive phytochemicals.

Keywords : Batrisu Vasanu; Galactagogue; Phytochemicals; GC-MS; Polyherbal.

CALL FOR PAPERS


Paper Submission Last Date
30 - November - 2025

Video Explanation for Published paper

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe