Authors :
Arvinder Pal Singh; Mona Piplani; Amit Aggarwal
Volume/Issue :
Volume 10 - 2025, Issue 8 - August
Google Scholar :
https://tinyurl.com/mr3jvym9
Scribd :
https://tinyurl.com/mr3ehajx
DOI :
https://doi.org/10.38124/ijisrt/25aug150
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Note : Google Scholar may take 30 to 40 days to display the article.
Abstract :
Colebrookea oppositifolia, a traditionally valued medicinal plant, was investigated for its phytochemical
constituents using different solvent extracts. The aerial parts of the plant were shade-dried, powdered, and subjected to
sequential extraction using n-hexane, chloroform, ethyl acetate, and methanol. Preliminary phytochemical screening
revealed a wide distribution of secondary metabolites, with methanol extract showing the highest diversity, including
alkaloids, flavonoids, tannins, saponins, phenolics, and glycosides. Chloroform and n-hexane fractions were particularly rich
in terpenoids and steroids, while ethyl acetate extract demonstrated moderate amounts of flavonoids and phenolic
compounds. These findings confirm that solvent polarity plays a critical role in the extraction of specific phytochemicals.
The results provide a scientific basis for the plant's traditional therapeutic applications and point to its potential as a source
of bioactive compounds for future pharmacological research. Further studies, including compound isolation and bioactivity
assays, are recommended to explore its full medicinal potential.
Keywords :
Colebrookea oppositifolia, Phytochemical Screening, Solvent Extraction, Secondary Metabolites, Alkaloids, Terpenoids.
References :
- Organization WH. WHO global report on traditional and complementary medicine 2019: World Health Organization; 2019.
- Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. The Scientific World Journal. 2017;2017(1):5873648.
- Peron G, Hošek J, Prasad Phuyal G, Raj Kandel D, Adhikari R, Dall’Acqua S. Comprehensive characterization of secondary metabolites from colebrookea oppositifolia (Smith) leaves from Nepal and assessment of cytotoxic effect and anti-Nf-κb and ap-1 activities in vitro. International Journal of Molecular Sciences. 2020;21(14):4897.
- Ambu G. Ethnobotanical and ethnopharmacological studies of medicinal plants used in rural areas of Kavrepalanchok District (Central Nepal). 2021.
- Kumar D, Singla RK, Sharma P, Kumar L, Kaur N, Dhawan RK, et al. Phytochemistry and polypharmacological potential of Colebrookea oppositifolia smith. Current Topics in Medicinal Chemistry. 2023;23(5):334-48.
- Martínez G, Mijares MR, De Sanctis JB. Effects of flavonoids and its derivatives on immune cell responses. Recent Patents on Inflammation & Allergy Drug Discovery. 2019;13(2):84-104.
- Shang Z, Sharma V, Kumar T, Dev K, Patil S. Phytochemical characterization and synergistic antibacterial effects of colebrookea oppositifolia essential oil as adjuvants to modern antibiotics in combating drug resistance. Drug Design, Development and Therapy. 2024:4601-14.
- Viswanatha GL, Shylaja H, Kumar HY, Venkataranganna M, Prasad N. Traditional uses, phytochemistry, and ethnopharmacology of Colebrookea oppositifolia Smith: a mini-review. Advances in Traditional Medicine. 2021;21(2):209-29.
- 9. Kajaria DK, Gangwar M, Sharma AK, Nath G, Tripathi YB, Tripathi J, et al. Comparative evaluation of phenol and flavonoid content of polyherbal drugs. Pharmacologyonline. 2011;3:1365-73.
- Harborne A. Phytochemical methods a guide to modern techniques of plant analysis: springer science & business media; 1998.
- Hossain MA, Al-Hdhrami SS, Weli AM, Al-Riyami Q, Al-Sabahi JN. Isolation, fractionation and identification of chemical constituents from the leaves crude extracts of Mentha piperita L grown in Sultanate of Oman. Asian Pacific journal of tropical biomedicine. 2014;4:S368-S72.
- Aly SH, Uba AI, Nilofar N, Majrashi TA, El Hassab MA, Eldehna WM, et al. Chemical composition and biological activity of lemongrass volatile oil and n-Hexane extract: GC/MS analysis, in vitro and molecular modelling studies. PloS one. 2025;20(2):e0319147.
- Bansode SS, Bharat MMP, Fathima MZ, Delgosha MS, Wagh JG, Hechhu R, et al. Evaluation of Invitro Methods of Antioxidant And Antiulcer Activity of Poly Herbal Plants From North India.
Colebrookea oppositifolia, a traditionally valued medicinal plant, was investigated for its phytochemical
constituents using different solvent extracts. The aerial parts of the plant were shade-dried, powdered, and subjected to
sequential extraction using n-hexane, chloroform, ethyl acetate, and methanol. Preliminary phytochemical screening
revealed a wide distribution of secondary metabolites, with methanol extract showing the highest diversity, including
alkaloids, flavonoids, tannins, saponins, phenolics, and glycosides. Chloroform and n-hexane fractions were particularly rich
in terpenoids and steroids, while ethyl acetate extract demonstrated moderate amounts of flavonoids and phenolic
compounds. These findings confirm that solvent polarity plays a critical role in the extraction of specific phytochemicals.
The results provide a scientific basis for the plant's traditional therapeutic applications and point to its potential as a source
of bioactive compounds for future pharmacological research. Further studies, including compound isolation and bioactivity
assays, are recommended to explore its full medicinal potential.
Keywords :
Colebrookea oppositifolia, Phytochemical Screening, Solvent Extraction, Secondary Metabolites, Alkaloids, Terpenoids.