Phytochemical Screening and Bioactive Compound Estimation of Colebrookea oppositifolia


Authors : Arvinder Pal Singh; Mona Piplani; Amit Aggarwal

Volume/Issue : Volume 10 - 2025, Issue 8 - August


Google Scholar : https://tinyurl.com/mr3jvym9

Scribd : https://tinyurl.com/mr3ehajx

DOI : https://doi.org/10.38124/ijisrt/25aug150

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Abstract : Colebrookea oppositifolia, a traditionally valued medicinal plant, was investigated for its phytochemical constituents using different solvent extracts. The aerial parts of the plant were shade-dried, powdered, and subjected to sequential extraction using n-hexane, chloroform, ethyl acetate, and methanol. Preliminary phytochemical screening revealed a wide distribution of secondary metabolites, with methanol extract showing the highest diversity, including alkaloids, flavonoids, tannins, saponins, phenolics, and glycosides. Chloroform and n-hexane fractions were particularly rich in terpenoids and steroids, while ethyl acetate extract demonstrated moderate amounts of flavonoids and phenolic compounds. These findings confirm that solvent polarity plays a critical role in the extraction of specific phytochemicals. The results provide a scientific basis for the plant's traditional therapeutic applications and point to its potential as a source of bioactive compounds for future pharmacological research. Further studies, including compound isolation and bioactivity assays, are recommended to explore its full medicinal potential.

Keywords : Colebrookea oppositifolia, Phytochemical Screening, Solvent Extraction, Secondary Metabolites, Alkaloids, Terpenoids.

References :

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Colebrookea oppositifolia, a traditionally valued medicinal plant, was investigated for its phytochemical constituents using different solvent extracts. The aerial parts of the plant were shade-dried, powdered, and subjected to sequential extraction using n-hexane, chloroform, ethyl acetate, and methanol. Preliminary phytochemical screening revealed a wide distribution of secondary metabolites, with methanol extract showing the highest diversity, including alkaloids, flavonoids, tannins, saponins, phenolics, and glycosides. Chloroform and n-hexane fractions were particularly rich in terpenoids and steroids, while ethyl acetate extract demonstrated moderate amounts of flavonoids and phenolic compounds. These findings confirm that solvent polarity plays a critical role in the extraction of specific phytochemicals. The results provide a scientific basis for the plant's traditional therapeutic applications and point to its potential as a source of bioactive compounds for future pharmacological research. Further studies, including compound isolation and bioactivity assays, are recommended to explore its full medicinal potential.

Keywords : Colebrookea oppositifolia, Phytochemical Screening, Solvent Extraction, Secondary Metabolites, Alkaloids, Terpenoids.

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Paper Submission Last Date
30 - November - 2025

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