The present study is design to evaluate preliminary phytochemical constituents, physiochemical properties evaluation, TLC & HPTLC fingerprinting analysis of Morus alba Linn (White mulberry) Leaves powder extraction. Total phenol content, total flavonoid content, Total tannin content, TLC, HPTLC fingerprinting analysis for compounds responsible for antioxidant activity. Phytochemical screening shows the presence of constituents like alkaloids, glycosides, carbohydrates & phytosterols, saponin, phenolic compounds, tannins, proteins, amino acids, gums and mucilage. Physicochemical studies shows that the powder was contain a moisture content 12.5% and ash value 7.5%. Total phenol content was 3.44 mg gallic acid equivalent/g, total flavonoid content was 5.00 mg rutin equivalent/g and total tannin content was 3.00 mg tannic acid equivalent/g. HPTLC fingerprinting also proofs the presence of phenol & flavonoid compounds in the leaf powder. The results in the paper shows that the Morus alba Linn (White mulberry) leaf powder extract is an natural antioxidant and can be used for treatment of various disorders.