Pilot testing was undertaken prior to a bioassay to be carried out using Tilapia Zillii as the test model which would be exposed to liquid habitats from River Challawa, Kano State, to access the differential bioaccumulation of heavy metals (Pb, Cr, Cd, Al, Zn, Cu and Fe) to liver and skeletal muscles. A pilot study is a reflection of the procedure of the main (large scale) study and is used to validate the feasibility of every part of the study protocol. From the physicochemical and heavy metals of the test liquid habitat and the control, all the parameters assayed for where above the maximum permissible limit set by Federal ministry of Environment for surface water, dissolved oxygen however was below 6.0 mg/L which is required for optimal metabolism of Tilapia zillii and subsequently, aerators were introduced to increase their chances of survival throughout the period of the bioassay. After the exposure for 24 hours to the test liquid habitat and control, liver and skeletal muscle tissues were analyzed for heavy metals and iron had the highest in both tissues and across the liquid habitats, with the concentration of 4.138 to 9.276 mg/kg for liver tissues across the liquid habitats and 2.812 to 6.857 mg/kg for skeletal muscle across the liquid habitats,. Finding of the pilot testing, indicate that the feasibility of the main (large scale) study is achievable with alteration in few areas such as: introduction of aerators and the duration

Keywords : Pilot Testing, Bioaccumulation, Feasibility, Heavy Metals.