Pineapple Peel Waste Juice as Low-Cost Fermentation Substrate for Bioethanol Production using Commercial Baker’s Yeast


Authors : Abdullah; Ainun Khoiriyah

Volume/Issue : Volume 8 - 2023, Issue 11 - November

Google Scholar : https://tinyurl.com/mr2au999

Scribd : https://tinyurl.com/564ckw7f

DOI : https://doi.org/10.5281/zenodo.10250927

Abstract : The energy currently being utilized comes from non-renewable sources, primarily fossil energy, which is anticipated to run out shortly. As a result, investigating alternate and sustainable energy sources is imperative, such as biofuels. Bioethanol, a biofuel derived from raw materials like lignocellulosic biomass from agricultural waste, is a promising option. It is produced through the fermentation reaction of sugars or polysaccharides facilitated by microorganisms. On an industrial scale, bioethanol is commonly produced from sugars obtained from basic starch products like cassava, corn or sugarcane. But the fact that these feedstocks— which are high in sugar and starch—are primarily utilized for food and feed has an impact on their steady supply. In Indonesia, pineapple production has been steadily increasing. Pineapple skin constitutes 41% of the total pineapple fruit, often discarded or used as animal feed. In 2022, pineapple production reached 3.2 million tons, with the potential for 1.3 million tons of pineapple peel waste. If not managed properly, this waste has the potential to pollute the environment. Pineapple peel waste (PPW) allows for the direct production of ethanol from the juice by requiring only a milling process to extract sugar into the fermentation medium. The characteristics and mineral composition of Pineapple peel waste (PPW) juice, along with the effects of adding nitrogen sources ((NH4)2SO4, (NH4)3PO4, (CO(NH2)2)) and micronutrient (ZnSO4·7H2O, CuSO4·7H2O, MnSO4·7H2O) were studied. It was discovered that the PPW juice had a 15.23% total sugar content. Utilizing Pineapple peel waste (PPW) juice as a raw material, bioethanol production was carried out using Baker’s yeast (Fermipan from Sangra Ratu Boga Company) with fermentation at 30°C for 72 hours at pH 5.5. The results revealed that the sample with urea supplementation (2.3595 g/l) produced the highest alcohol content at 13.052%, while the sample with Copper Sulfate supplementation (0.05 g/l) yielded 11.723% alcohol. These findings underscore the potential for sugar production from Pineapple peel waste (PPW) for subsequent bioethanol production. Further optimization of bioreactor parameters is required to enhance bioethanol production.

Keywords : Bioethanol; Fermentation; Pineapple Peel; Waste; Baker’s Yeast; Nutrient; Renewable Sugars.

The energy currently being utilized comes from non-renewable sources, primarily fossil energy, which is anticipated to run out shortly. As a result, investigating alternate and sustainable energy sources is imperative, such as biofuels. Bioethanol, a biofuel derived from raw materials like lignocellulosic biomass from agricultural waste, is a promising option. It is produced through the fermentation reaction of sugars or polysaccharides facilitated by microorganisms. On an industrial scale, bioethanol is commonly produced from sugars obtained from basic starch products like cassava, corn or sugarcane. But the fact that these feedstocks— which are high in sugar and starch—are primarily utilized for food and feed has an impact on their steady supply. In Indonesia, pineapple production has been steadily increasing. Pineapple skin constitutes 41% of the total pineapple fruit, often discarded or used as animal feed. In 2022, pineapple production reached 3.2 million tons, with the potential for 1.3 million tons of pineapple peel waste. If not managed properly, this waste has the potential to pollute the environment. Pineapple peel waste (PPW) allows for the direct production of ethanol from the juice by requiring only a milling process to extract sugar into the fermentation medium. The characteristics and mineral composition of Pineapple peel waste (PPW) juice, along with the effects of adding nitrogen sources ((NH4)2SO4, (NH4)3PO4, (CO(NH2)2)) and micronutrient (ZnSO4·7H2O, CuSO4·7H2O, MnSO4·7H2O) were studied. It was discovered that the PPW juice had a 15.23% total sugar content. Utilizing Pineapple peel waste (PPW) juice as a raw material, bioethanol production was carried out using Baker’s yeast (Fermipan from Sangra Ratu Boga Company) with fermentation at 30°C for 72 hours at pH 5.5. The results revealed that the sample with urea supplementation (2.3595 g/l) produced the highest alcohol content at 13.052%, while the sample with Copper Sulfate supplementation (0.05 g/l) yielded 11.723% alcohol. These findings underscore the potential for sugar production from Pineapple peel waste (PPW) for subsequent bioethanol production. Further optimization of bioreactor parameters is required to enhance bioethanol production.

Keywords : Bioethanol; Fermentation; Pineapple Peel; Waste; Baker’s Yeast; Nutrient; Renewable Sugars.

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