Authors : Princess Janina D. Anggulo; Railyn L. Daud; Wagne Dale J. Garcesa; Juhaina T. Muripaga

Volume/Issue : Volume 11 - 2026, Issue 7 - July

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Google Scholar : https://tinyurl.com/yc2jbn5m

Scribd : https://tinyurl.com/z9rndhhn

DOI : https://doi.org/10.38124/ijisrt/26jul418

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Abstract : This study were investigated the effectiveness of S. jamaicensis root for wound healing. Despite the plant’s historical use in traditional medicine practice, scientific evidence were limited, particularly concerning the root. To address this, the researchers evaluated the methanolic root extract's efficacy in promoting wound closure and tissue regeneration in an animal model. The extract, prepared by macerating dried root using methanol, were applied topically to excised wounds in male albino mice. Results were compared to a positive control, Povidone Iodine, and a negative control, Base formulation.

Keywords : Root Crude Extract of S. jamaicensis, Povidone Iodine, Excision Model.

References :

• Journals

1. Alemu BK, Ayalew Getahun K, Kahaliw w. <p>in vitro antioxidant and in vivo wound healing activities of the 80% methanol extract and solvent fractions of seeds of<em>brassica (Brassicaceae) in mice</p>. Journal of Experiment Pharmacology. 2020; Volume 12: 463-474.doi:10.2147/jep. s278622
2. Alherz FA, Negm WA, Elekhnawy E, El-Masry TA, Haggag EM, Alqahtani MJ, Hussein IA. Silver nanoparticles prepared using Encephalartos Laurentianus de wild leaf extract have inhibitory activity against candida albicans clinical isolates. Journal of Fungi. 2022;8(10):1005. doi:10.3390/jof8101005
3. Belachew TF, Asrade S, Geta M, Fentahun E. In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of 80% Methanol Crude Flower Extract of Hagenia abyssinica (Bruce) J.F. Gmel in Mice. Evid Based Complement Alternat Med. 2020 Mar 30;2020:9645792. doi: 10.1155/2020/9645792. PMID: 32308725; PMCID: PMC7149342.
4. Bigliardi PL, Alsagoff SA, El-Kafrawi HY, Pyon J-K, Wa CT, Villa MA. Povidone iodine in wound healing: A review of current concepts and practices. International Journal of Surgery. 2017;44:260–268. doi:10.1016/j.ijsu.2017.06.073
5. Chokotho L, van Hasselt E. The use of tannins in the local treatment of burn wounds - a pilot study. Malawi Med J. 2005 Jun;17(1):19-20. doi: 10.4314/mmj.v17i1.10866. PMID: 27528993; PMCID: PMC3346037.
6. G/Giorgis SG, Ambikar D, Tsegaw A, Belayneh YM. Wound Healing Activity of 80% Methanolic Crude Extract and Solvent Fractions of the Leaves of Justicia schimperiana (Hochst. ex Nees) T. Anderson (Acanthaceae) in Mice. J Exp Pharmacol. 2022 May 13;14:167-183. doi: 10.2147/JEP.S340177. PMID: 35592645; PMCID: PMC9113456.
7. Harun H, Haroen H, Mirwanti R, Apriani N, Akuoko C. Uncovering the benefits of Povidone Iodine compared to other therapeutic agents in wound infection prevention and healing outcomes: A scoping review. Journal of Multidisciplinary Healthcare. 2024;Volume 17:3605–3616. doi:10.2147/jmdh.s469037
8. Hilip Asumang, Yaw Duah Boakye, Theresa Appiah Agana, Jibira Yakubu, Philomena Entsie, William Gariba Akanwariwiak, Francis Adu, Christian Agyare.2021.Antimicrobial, antioxidant and wound healing activities of methanol leaf extract of Bridelia micrantha (Hochst.) Baill,Scientific African,Volume 14,e00980,ISSN 2468-2276,https://doi.org/10.1016/j.sciaf.2021.e00980.
9. Hora AB, Biano LS, Nascimento AC, Camargo ZT, Heiden GI, Albulquerque-Júnior RL, Grespan R, Aragão JM, Camargo EA. Isoorientin improves excisional skin wound healing in mice. Pharmaceuticals. 2024;17(10):1368. doi:10.3390/ph17101368
10. Kancherla N, Dhakshinamoothi A, Chitra K, Komaram RB.2019. Prelimiminary analysis ofphytoconstituents and evaluation of anthekminthic property of Cayratia auriculata(in        vitro).     Medica-A Journal of Clinical Medicine.14(4).doi:10.26574/maedica.2019.14.4.350
11. Kuhlmann M, Wigger-Alberti W, Mackensen Y v., Ebbinghaus M, Williams R, KrauseKyora F, Wolber R. Wound healing characteristics of a novel wound healing ointment in an abrasive wound model: A randomised, intra-individual clinical investigation. Wound Medicine. 2019;24(1):24–32. doi:10.1016/j.wndm.2019.02.002
12. Masson‐Meyers DS, Andrade TA, Caetano GF, Guimaraes FR, Leite MN, Leite SN, Frade MA. Experimental models and methods for cutaneous wound healing assessment. International Journal of Experimental Pathology. 2020;101(1–2):21–37. doi:10.1111/iep.12346
13. Marcin Wekwejt, Marcin Małek, Anna Ronowska, Anna Michno, Anna Pałubicka, Lidia Zasada, Agnieszka Klimek, Beata Kaczmarek-Szczepańska.2024.Hyaluronic acid/tannic acid films for wound healingapplication,InternationalJournalofBiological Macromolecules,Volume254,Part3,128101,ISSN01418130,https://doi.org/10.1016/j.ij biomac.2023.128101.
14. Macmillan.SciRep.2016.Jul11;6:29707.doi:10.1038/srep29707https://pmc.ncbi.nlm.ni h.gov/articles/PMC4942614/figure/f4/
15. Ovinuchi Ejiohuo, Samson O. Folami, Deinmo Edi, Jessica Isaac.2024.Polyphenol encapsulated nanofibers in wound healing and drug delivery,European Journal of MedicinalChemistryReports,Volume12,100184,ISSN27724174,https://doi.org/10.101 6/j.ejmcr.2024.100184
16. Rowland MB, Moore PE, Bui C, Correll RN. Assessing wound closure in mice using skin-punch biopsy. STAR Protocols. 2023;4(1): 101989.doi: 10.1016/j.xpro.2022.101989
17. Shaikh, Junaid & Patil, Matsyagandha. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies. 8. 603-608. 10.22271/chemi.2020.v8.i2i.8834.
18. Yadav PD, Modi K, Shah M. 2022. Phytochemistry, pharmacology, and botanical aspects of Stachytarpheta species: a review. Int J Green Pharm [journal]. 15:114. doi:10.22377/ijgp.v15i2.3078.
19. Yamamoto K, Miwa S, Yamada T, Setozaki S, Hamuro M, Kurokawa S, Enomoto S. Major impact of moist wound healing on autologous tissue regeneration: A review of ulcer treatment. Health Sci Rep. 2022 Dec 31;6(1):e1029. doi: 10.1002/hsr2.1029. PMID: 36605455; PMCID: PMC9804439.
20. Yiblet TG, Tsegaw A, Ahmed N, Dagnew SB, Tadesse TY, Kifle ZD. Evaluation of wound healing activity of 80% and solvent fractions of Stephania abyssinica (dill. & A. rich). Walp. (Menispermaceae) in mice. Journal of Experimental Pharmacology. 2022; Volume 14: 225-273.doi:10.2147/jep. s364282
21. Zulkefli N, Che Zahari CN, Sayuti NH, Kamarudin AA, Saad N, Hamezah HS, Bunawan H, Baharum SN, Mediani A, Ahmed QU, et al. Flavonoids as potential wound-healing molecules: Emphasis on Pathways Perspective. International Journal of Molecular Sciences. 2023;24(5):4607. doi:10.3390/ijms24054607

• Books

22. Grubbs H. Wound physiology. StatPearls [Internet]. 2023 May 16 [accessed 2024 Nov 22]. https://www.ncbi.nlm.nih.gov/books/NBK518964/
23. Herman TF. Wound classification. StatPearls [Internet]. 2023 Aug 17 [accessed 2024 Nov 22]. https://www.ncbi.nlm.nih.gov/books/NBK554456/

• Websites

24. Cologne, Germany: Institute for Quality and Efficiency in Health Care (IQWiG); 2006-. Overview: Chronic wounds. [Updated 2022 Aug 8]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK326431/
25. Stachytarpheta jamaicensis (Jamaica vervain). CABI Compendium.2022 Jan 7. doi:10.1079/cabicompendiem.51364
26. Fatmawati S, Auwaliyah F, Yuliana, Hasanah N, Putri DA, Kainama H, Choudhary MI. Antioxidant and α-glucosidase inhibitory activities of compound isolated from
27. Stachytarpheta jamaicensis (L) vahl. leaves. Nature News. 2023 Oct 30 [accessed 2024 Nov 22]. https://www.nature.com/articles/s41598-023-45357-z
28. Jacela JJ, Rello AV, Bermudo NI. 2021. Formulation and evaluation of antibacterial gelincorporated...[accessed2024Nov21].
29. http://www.publiscience.org/wpcontent/uploads/2021/08/Formulation-of-an- antibacterial-gel-incorporated-with-S.jamaicensis-extract-1.pdf
30. Jennifer Ragi MD,a Amy Pappert MD,a Babar Rao MD,a Daphna Havkin-Frenkel PhD,b Sandy Milgraum MD.2011.Oregano Extract Ointment for Wound Healing: A
31. Randomized, Double-Blind, Petrolatum-Controlled Study Evaluating Efficacy. Volume10.https://jddonline.com/articles/oregano-extract-ointment-for-wound-healing- a-randomized-double-blind-petrolatum-controlled-study-eva-S1545961611P1168X
32. Kamrani P, Hedrick J, Marks JG, Zaenglein AL. 2024. Petroleum jelly: A comprehensive review of its history, uses, and safety. J Am Acad Dermatol. 90 https://www.sciencedirect com/science/article/abs/pii/S0190962223011076
33. Mohamed and Seleem. Drug Des Devel Ther.2014.Oct17;8:1979–1983 doi:10.2147/DDDT.S72129 [PDF] wound healing potential of the crude leaf extract of Stachytarpheta Jamaicensis Linn. Vahl (Kandikandilaan) on induced wounds in rats | semantic scholar. [accessed 2024 Nov 21]. https://www.semanticscholar.org/paper/Wound-healing-potential-ofthe- crude-leaf-extract-Caluya/b6cc653cd847093fee79ccdfb9b5e884d7163cb3
34. Zheng Y. and Wen Y. 2013.Aerobic exercise equipment and device and application methodthereof.CNpatentCN102885852A.https://patents.google.com/patent/CN102885852A/e
35. Zhang Q, Kong L, Wang Q, Wang H, Yang Y, Fu J, Zhang Y, Dong J, Zeng C, Liu H. A biotin-stabilized HKUST-1/ADM scaffold for facilitating MSC endothelial differentiation and vascularization in diabetic wound healing. Biomaterials Science. 2022 Dec 14 [accessed2025Apr30].https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm01443b/unauth#
36. Criollo-Mendoza MS;Contreras-Angulo LA;Leyva-López N;Gutiérrez-Grijalva EP;Jiménez-Ortega LA;Heredia JB; Wound healing properties of natural products: Mechanisms of action. Molecules (Basel, Switzerland). [accessed 2025 May 30]. https://pubmed.ncbi.nlm.nih.gov/36677659/
37. OECD/OCDE 402. [accessed 2025 May 30]. https://www.oecd.org/content/dam/oecd/en/publications/reports/2017/10/test-no-402-acute-dermal-toxicity_g1gh2925/9789264070585-en.pdf
38. CN102885852A - povidone iodine ointment and preparation method thereof. Google Patents.  [accessed 2025 May 30]. https://patents.google.com/patent/CN102885852A/en