Production of Protein Hydrolysate from Broken Rice by Crude Bromelain Enzyme in Different Conditions


Authors : Zalniati Fonna Rozali, Nurheni Sri Palupi, Endang Yuli Purwani, Maggy Thenawidjaja Suhartono.

Volume/Issue : Volume 4 - 2019, Issue 6 - June

Google Scholar : https://goo.gl/DF9R4u

Scribd : https://bit.ly/2XJM03s

Broken rice is a by-product of low-value rice mills in Indonesia, a country where pineapples are also produced abundantly. The proteins derived from broken rice, along with bromelin enzymes from pineapples can potentially be used in the production of their vegetable hydrolyzates, the demand of which has been increasing lately. This study was designed to determine the conditions suitable for the production of rice protein hydrolyzates from broken rice using crude bromelain enzyme. This study used flour from broken rice and its protein isolates as substrate in the hydrolysis process while using the crude extracts of bromelain enzyme as a source of protease. In the initial stage, bromelain enzyme activity was measured at pHs of 3, 4, 5, 6, 7 and 8. Furthermore, rice flour and its protein isolate were hydrolyzed by crude bromelin enzymes at a selected pH for 1 and 3 hours with the changes in protein profile later being analyzed at 1 and 3 hours after hydrolysis.

Keywords : Rice; Bromelain, Hydrolysis, Protein; Intensity.

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