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Protective Role of Salivary Proteins in Dental Erosion: Biochemical Perspectives


Authors : Aksu Samet; Akgul Omeragic; Sezen Samet

Volume/Issue : Volume 11 - 2026, Issue 6 - June


Google Scholar : https://tinyurl.com/fvza6sz4

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DOI : https://doi.org/10.38124/ijisrt/26jun1289

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Abstract : Smoking history and frequency of reflux were also evaluated. The participants with dental erosion had significantly lower values of total proteins, calcium and pH compared to the control group, in both unstimulated and stimulated saliva, indicating an unfavorable biochemical environment for enamel stability. More patients with dental erosion smoke than do patients without dental erosion, and the frequency of regurgitation was different. The combination of decreased calcium and total proteins in saliva indicates a disturbance in the biochemical mechanisms that maintain enamel integrity. The protein depletion presumably contributes to a lowered dielectric strength against erosive challenges, when we consider the established functions of the salivary proteins in the formation of the acquired pellicle, the regulation of mineral ion concentrations, and the protection of surfaces. The data suggest that dental erosion is due to a multi-factorial biochemical imbalance in saliva, involving alterations of acid - base states, decreased availability of minerals, and decreased protective capabilities of proteins, which increases the susceptibility of enamel to erosive destruction.

Keywords : Sakiva Porteins; Calcium; Saliva pH; Smoking; Dental Erosion.

References :

  1. Edgar, M., Dawes, C., & O'Mullane, D. Saliva and oral health (4th ed.). Stephen Hancocks Limited., 2012. https://stage.wrigleyoralcare.com/s3media/2022-02/SHL_S_OH_A5_2015_FINAL.pdf
  2. Dawes, C., Pedersen, A. M. L., Villa, A., Ekström, J., Proctor, G. B., Vissink, A., Aframian, D., McGowan, R., Aliko, A., Narayana, N., Sia, Y. W., Joshi, R. K., Jensen, S. B., Kerr, A. R., & Wolff, A. The functions of human saliva: A review sponsored by the World Workshop on Oral Medicine VI. Archives of Oral Biology, 60(6), 863–874, 2015.  https://doi.org/10.1016/j.archoralbio.2015.03.004
  3. Carpenter, G. H. The secretion, components, and properties of saliva. Annual Review of Food Science and Technology, 4, 267–276, 2013. https://doi.org/10.1146/annurev-food-030212-182700
  4. Hannig, M., & Hannig, C. The pellicle and erosion. In A. Lussi (Ed.), Monographs in Oral Science, Vol. 25, pp. 206–214, 2014. Karger. https://doi.org/10.1159/000360376
  5. Fischer, N. G., & Aparicio, C. The salivary pellicle on dental biomaterials. Colloids and Surfaces B: Biointerfaces, 200, 111570, 2021. https://doi.org/10.1016/j.colsurfb.2021.111570
  6. Castagnola, M., Cabras, T., Iavarone, F., Fanali, C., Nemolato, S., Peluso, G., Bosello, S. L., Faa, G., Ferraccioli, G., & Messana, I. The human salivary proteome: A critical overview of the results obtained by different proteomic platforms. Expert Review of Proteomics, 9(1), 33–46, 2012.  https://doi.org/10.1586/EPR.11.77
  7. Vukosavljevic, D., Custodio, W., Buzalaf, M. A. R., & Hara, A. T. Salivary proteins as predictors and controls for oral health. Journal of Cellular Physiology, 226(10), 2356–2360, 2011.  https://doi.org/10.1002/jcp.22615
  8. Hara, A. T., & Zero, D. T. The caries environment: Saliva, pellicle, diet, and hard tissue ultrastructure. Dental Clinics of North America, 54(3), 455–467,2010.  https://doi.org/10.1016/j.cden.2010.03.008
  9. Lussi, A., Schlueter, N., Rakhmatullina, E., & Ganss, C. Dental erosion—An overview with emphasis on chemical and histopathological aspects. Caries Research, 45(Suppl. 1), 2–12, 2011. https://doi.org/10.1159/000325915
  10. Lussi, A., & Carvalho, T. S. Erosive tooth wear: A multifactorial condition of growing concern and increasing knowledge. In A. Lussi & C. Ganss (Eds.), Erosive tooth wear: From diagnosis to therapy 25, 1–15, 2014 https://doi.org/10.1159/000360380
  11. Martínez, L. M., Lietz, L. L., Tarín, C. C., García, C. B., Tormos, J. I. A., & Miralles, E. G. Analysis of the pH levels in energy and pre-workout beverages and frequency of consumption: A cross-sectional study. BMC Oral Health, 24(1), 1082.2024. https://doi.org/10.1186/s12903-024-04843-0
  12. Schulze, A., & Busse, M. Sports diet and oral health in athletes: A comprehensive review. Medicina, 60(2), 319, 2024. https://doi.org/10.3390/medicina60020319
  13. Samet, A., Omeragic, A., Velkovski, V., & Samet, S. Determination of the pH value of unstimulated and stimulated saliva in a group of patients with and without dental erosion. Journal of Natural Sciences and Mathematics of UT, 10(19–20), 17–30, 2025. https://doi.org/10.62792/ut.jnsm.v10.i19-20.p3106
  14. Samet, A., Omeragic, A., Velkovski, V., Samet, S., & Pechijareva Sadikarijo, I. Dietary acidity and dental erosion: Effects of frequent acidic food and beverage intake. International Journal of Technical and Natural Sciences, 5(2), 71–86, 2025. https://doi.org/10.69648/GLJY3848
  15. Omeragić, A. Dejstvoto na nadvorešnite i vnatrešnite faktori vrz nekarioznite leziji.Master's thesis, Ss. Cyril and Methodius University in Skopje, Faculty of Dentistry, 2016. https://stomfak.ukim.edu.mk/books/page/4/?wbg_author_s=Омераѓиќ%20Акѓул
  16. Navazesh, M., & Kumar, S. (2008). Measuring salivary flow. The Journal of the American Dental Association, 139(Suppl.), 35S–40S, 2008. https://pubmed.ncbi.nlm.nih.gov/18460678/
  17. Bianchi-Bosisio, A. Proteins: Physiological samples. In P. Worsfold, A. Townshend, & C. Poole (Eds.), Encyclopedia of analytical science (2nd ed., pp. 357–375). Elsevier., 2005. https://doi.org/10.1016/B0-12-369397-7/00494-5
  18. Goldring, J. P. D. Spectrophotometric methods to determine protein concentration. In B. Kurien & R. Scofield (Eds.), Western blotting (Methods in Molecular Biology, Vol. 1312, pp. 41–47). Humana Press., 2015. https://doi.org/10.1007/978-1-4939-2694-7_7
  19. Samet, A. Spectrophotometric monitoring of the oxidation of thiobenzamide at varying oxidant concentrations. KNOWLEDGE – International Journal, 73(3), 583–588, 2025. https://ojs.ikm.mk/index.php/kij/article/view/7943
  20. Aguiar, J. I. S., Rangel, A. O. S. S., & Mesquita, R. B. R. Salivary calcium determination with a specially developed microfluidic paper-based device for point-of-care analysis. Talanta Open, 8, 100254, 2023. https://doi.org/10.1016/j.talo.2023.100254
  21. Narasimman, N., Nagarajan, S., Krishnaswamy, V., Desingu, V., Deepa, V. M., & Meikandan, G. Analysis of salivary pH in healthy and diseased periodontium among young and elderly adults. Journal of Pharmacy & Bioallied Sciences, 17(Suppl. 3), S2680–S2682, 2025.https://doi.org/10.4103/jpbs.jpbs_1179_25
  22. Deepa, T., & Thirrunavukkarasu, N. Saliva as a potential diagnostic tool. Indian Journal of Medical Sciences, 64(7), 293–306, 2010. https://doi.org/10.4103/0019-5359.99854
  23. Baumann T, Carvalho TS, Lussi A. The effect of enamel proteins on erosion. Sci Rep. 15, 5, 15194,2015. https://doi.org/10.1038/srep15194
  24. Dodds, M., Roland, S., Edgar, M., & Thornhill, M.. Saliva: A review of its role in maintaining oral health and preventing dental disease. BDJ Team, 2, Article 15123, 2015. https://doi.org/10.1038/bdjteam.2015.123
  25. Hase, P., Shah, V., Gunjal, S., Manjunatha, B. S., & Pateel, D. G. S. Dental calculus deposition: Correlation with salivary statherin and calcium levels. International Journal of Dentistry, 2026, 5356016, 2026. https://doi.org/10.1155/ijod/5356016
  26. Saha A, Kim Y, Kim KK, Kim YJ, Byon HR, Hong S. Nanoscale Study on Noninvasive Prevention of Dental Erosion of Enamel by Silver Diamine Fluoride. Biomater. Res. 28: 0103.2024. https://doi.org/10.34133/bmr.0103
  27. Schestakow, A., Schmitt, V., Câmara, J. V. F., & Hannig, M.. Infiltration of salivary proteins into dentin during erosive processes. Journal of Dentistry, 152, 105457.2025. https://doi.org/10.1016/j.jdent.2024.105457

Smoking history and frequency of reflux were also evaluated. The participants with dental erosion had significantly lower values of total proteins, calcium and pH compared to the control group, in both unstimulated and stimulated saliva, indicating an unfavorable biochemical environment for enamel stability. More patients with dental erosion smoke than do patients without dental erosion, and the frequency of regurgitation was different. The combination of decreased calcium and total proteins in saliva indicates a disturbance in the biochemical mechanisms that maintain enamel integrity. The protein depletion presumably contributes to a lowered dielectric strength against erosive challenges, when we consider the established functions of the salivary proteins in the formation of the acquired pellicle, the regulation of mineral ion concentrations, and the protection of surfaces. The data suggest that dental erosion is due to a multi-factorial biochemical imbalance in saliva, involving alterations of acid - base states, decreased availability of minerals, and decreased protective capabilities of proteins, which increases the susceptibility of enamel to erosive destruction.

Keywords : Sakiva Porteins; Calcium; Saliva pH; Smoking; Dental Erosion.

Paper Submission Last Date
31 - July - 2026

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