Authors :
Elavarasi.E; Dr. R.Srinivasan
Volume/Issue :
Volume 8 - 2023, Issue 3 - March
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/3Ujmixi
DOI :
https://doi.org/10.5281/zenodo.7807375
Abstract :
In the present situation there is an number of
death is cancer with 19.3 million deaths. Some usual
therapies are immune therapy, chemotherapy, and
radiotherapy for cancer. Through this therapy treatment
strategies may yield even better outcomes. A redox
protein is Azurin having 128 amino acids with a size of
14kDa and secreted by Pseudomonas aeruginosa as a
periplasmic protein. And the family is cupredoxin.
Azurin's anticancer properties have demonstrated that
when it interacts with P53, more intracellular
transcription factors are produced and P53 is stabilized.
The idea of developing high-quality pharmaceutical
goods with quality in mind (QbD) has replaced it. It is a
significant component of the current trend in
pharmaceutical quality; QbD is the best way to produce
high-quality, comprehensive pharmaceutical products,
but it also poses a significant challenge to the sector's
historically established procedures. The C-terminal
domain of azurin has four sections that make up the
Alpha helix: CD, GH, FG, and EF. A compound can be
measured using several methods and identified by
different methods of analysis. High extraction is a
higher-pressure analytical technique that can be used to
conduct a more thorough investigation of medicinal
products (HPLC), and liquid chromatography-mass
spectrometry (LC-MS/MS). Several extraction methods
can be used to evaluate drugs, including liquid-liquid
extraction. Adding a combination drug is known as
Dabrafenib. It is an anti-cancer medication used for the
treatment of cancer. Its purity and dependability are
ensured by using approaches like validation and
analytical tests. Using MODDE software version 8.2,
DOE(Design of experiment)was statistically designed
(Umetrics Inc., NJ, USA). Responsive surface modeling
(RSM) was used to calculate the non-linear
multidimensional relationship between the independent
components and CQAs.
Keywords :
Dabrafenib, Azurin, LC-MS/MS, Pseudomonas aeruginosa
In the present situation there is an number of
death is cancer with 19.3 million deaths. Some usual
therapies are immune therapy, chemotherapy, and
radiotherapy for cancer. Through this therapy treatment
strategies may yield even better outcomes. A redox
protein is Azurin having 128 amino acids with a size of
14kDa and secreted by Pseudomonas aeruginosa as a
periplasmic protein. And the family is cupredoxin.
Azurin's anticancer properties have demonstrated that
when it interacts with P53, more intracellular
transcription factors are produced and P53 is stabilized.
The idea of developing high-quality pharmaceutical
goods with quality in mind (QbD) has replaced it. It is a
significant component of the current trend in
pharmaceutical quality; QbD is the best way to produce
high-quality, comprehensive pharmaceutical products,
but it also poses a significant challenge to the sector's
historically established procedures. The C-terminal
domain of azurin has four sections that make up the
Alpha helix: CD, GH, FG, and EF. A compound can be
measured using several methods and identified by
different methods of analysis. High extraction is a
higher-pressure analytical technique that can be used to
conduct a more thorough investigation of medicinal
products (HPLC), and liquid chromatography-mass
spectrometry (LC-MS/MS). Several extraction methods
can be used to evaluate drugs, including liquid-liquid
extraction. Adding a combination drug is known as
Dabrafenib. It is an anti-cancer medication used for the
treatment of cancer. Its purity and dependability are
ensured by using approaches like validation and
analytical tests. Using MODDE software version 8.2,
DOE(Design of experiment)was statistically designed
(Umetrics Inc., NJ, USA). Responsive surface modeling
(RSM) was used to calculate the non-linear
multidimensional relationship between the independent
components and CQAs.
Keywords :
Dabrafenib, Azurin, LC-MS/MS, Pseudomonas aeruginosa
