QbD Approach in the Comparative Study of Azurin and Dabrafenib Anticancer Agents by UPLC Mass Septrometry


Authors : Elavarasi.E; Dr. R.Srinivasan

Volume/Issue : Volume 8 - 2023, Issue 3 - March

Google Scholar : https://bit.ly/3TmGbDi

Scribd : https://bit.ly/3Ujmixi

DOI : https://doi.org/10.5281/zenodo.7807375

Abstract : In the present situation there is an number of death is cancer with 19.3 million deaths. Some usual therapies are immune therapy, chemotherapy, and radiotherapy for cancer. Through this therapy treatment strategies may yield even better outcomes. A redox protein is Azurin having 128 amino acids with a size of 14kDa and secreted by Pseudomonas aeruginosa as a periplasmic protein. And the family is cupredoxin. Azurin's anticancer properties have demonstrated that when it interacts with P53, more intracellular transcription factors are produced and P53 is stabilized. The idea of developing high-quality pharmaceutical goods with quality in mind (QbD) has replaced it. It is a significant component of the current trend in pharmaceutical quality; QbD is the best way to produce high-quality, comprehensive pharmaceutical products, but it also poses a significant challenge to the sector's historically established procedures. The C-terminal domain of azurin has four sections that make up the Alpha helix: CD, GH, FG, and EF. A compound can be measured using several methods and identified by different methods of analysis. High extraction is a higher-pressure analytical technique that can be used to conduct a more thorough investigation of medicinal products (HPLC), and liquid chromatography-mass spectrometry (LC-MS/MS). Several extraction methods can be used to evaluate drugs, including liquid-liquid extraction. Adding a combination drug is known as Dabrafenib. It is an anti-cancer medication used for the treatment of cancer. Its purity and dependability are ensured by using approaches like validation and analytical tests. Using MODDE software version 8.2, DOE(Design of experiment)was statistically designed (Umetrics Inc., NJ, USA). Responsive surface modeling (RSM) was used to calculate the non-linear multidimensional relationship between the independent components and CQAs.

Keywords : Dabrafenib, Azurin, LC-MS/MS, Pseudomonas aeruginosa

In the present situation there is an number of death is cancer with 19.3 million deaths. Some usual therapies are immune therapy, chemotherapy, and radiotherapy for cancer. Through this therapy treatment strategies may yield even better outcomes. A redox protein is Azurin having 128 amino acids with a size of 14kDa and secreted by Pseudomonas aeruginosa as a periplasmic protein. And the family is cupredoxin. Azurin's anticancer properties have demonstrated that when it interacts with P53, more intracellular transcription factors are produced and P53 is stabilized. The idea of developing high-quality pharmaceutical goods with quality in mind (QbD) has replaced it. It is a significant component of the current trend in pharmaceutical quality; QbD is the best way to produce high-quality, comprehensive pharmaceutical products, but it also poses a significant challenge to the sector's historically established procedures. The C-terminal domain of azurin has four sections that make up the Alpha helix: CD, GH, FG, and EF. A compound can be measured using several methods and identified by different methods of analysis. High extraction is a higher-pressure analytical technique that can be used to conduct a more thorough investigation of medicinal products (HPLC), and liquid chromatography-mass spectrometry (LC-MS/MS). Several extraction methods can be used to evaluate drugs, including liquid-liquid extraction. Adding a combination drug is known as Dabrafenib. It is an anti-cancer medication used for the treatment of cancer. Its purity and dependability are ensured by using approaches like validation and analytical tests. Using MODDE software version 8.2, DOE(Design of experiment)was statistically designed (Umetrics Inc., NJ, USA). Responsive surface modeling (RSM) was used to calculate the non-linear multidimensional relationship between the independent components and CQAs.

Keywords : Dabrafenib, Azurin, LC-MS/MS, Pseudomonas aeruginosa

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