Quantification of Thymoquinone (TQ) and Antioxidant Properties in Hydro -ethanolic extract of Nigella sativa

Authors : Saliya. S. Bawani; D. Usha Anandhi

Volume/Issue : Volume 6 - 2021, Issue 12 - December

Google Scholar : http://bitly.ws/gu88

Scribd : https://bit.ly/3rerO6g

Nigella sativa L. belongs to family Ranuculaceae family, is an annual herbaceous plant which is found in the Middle East, Eastern Europe, Western Asia, and Central Asia. Because of its pharmacological and neuro protective properties, this plant has been largely studied in recent years. The goal of this study is to look at the antioxidant properties of Nigella sativa seeds hydro-ethanolic extract. N.sativa seeds were soxhlet extracted in 70% ethanol (v/v) for 48 hours at 50C°. DPPH, hydroxyl radical, and singlet oxygen testing methods were used to estimate antioxidant capabilities. Using HPLC, the presence and concentration of Thymoquinone, a bioactive component, was also determined in the hydro-ethanolic extract. The main component found in the N. sativa seed extract was shown to have exceptional antioxidant capabilities. As a result, it can be regarded an important agent in nutrition, pharmaceuticals, and the treatment of oxidative stress.

Keywords : Nigella sativa, Hydro-ethanolic extract, Thymoquinone, Antioxidant properties, HPLC


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