Authors :
Nirmal Kumar Sinha; Gautam Kumar
Volume/Issue :
Volume 5 - 2020, Issue 10 - October
Google Scholar :
http://bitly.ws/9nMw
Scribd :
https://bit.ly/2Taf9B2
Abstract :
Rauwolfia serpentina L Benth Kurz, common
name Sarpgandha is a small perennial medicinal shrub
of family Apocynaceae. It has a vast array of secondary
metabolites, which have a number of commercial
applications. The present investigation is an effort to
developing an efficient protocol for callus induction
using various explants of the same. Callus induction was
obtained both from leaf as well as stem explants. It is
noteworthy that in the present investigation callus
formation from seed explants was also observed in MS
basal media (Plate 2). Two different combinations of
hormones i.e.2, 4-D + BAP, IAA + BAP, IBA+BAP &
Kinetin+NAA with different concentrations were tried
for callus induction. In the present research work BAP
alone induced callusing in both the explants i.e. leaf and
stem, which was 91.54% & 88.83% respectivel
Keywords :
Rauwolfia serpentina, medicinal plant, micropropagation, phytohormone, tissue culture.
Rauwolfia serpentina L Benth Kurz, common
name Sarpgandha is a small perennial medicinal shrub
of family Apocynaceae. It has a vast array of secondary
metabolites, which have a number of commercial
applications. The present investigation is an effort to
developing an efficient protocol for callus induction
using various explants of the same. Callus induction was
obtained both from leaf as well as stem explants. It is
noteworthy that in the present investigation callus
formation from seed explants was also observed in MS
basal media (Plate 2). Two different combinations of
hormones i.e.2, 4-D + BAP, IAA + BAP, IBA+BAP &
Kinetin+NAA with different concentrations were tried
for callus induction. In the present research work BAP
alone induced callusing in both the explants i.e. leaf and
stem, which was 91.54% & 88.83% respectivel
Keywords :
Rauwolfia serpentina, medicinal plant, micropropagation, phytohormone, tissue culture.
