Kohl is a revolutionary technique and a novel are conceived of as the production of Kohl herbal kajal with medicinal plants for enhancement. The key benefits of these Cosmetics items are greater patient conformity, waterresistant properties, durability and cost-efficient shaping Curve. Revealed the values were within the prescribed limits by standardization of the herbs was performed based on different physiochemical parameters. On the basis of selected parameters and its anti-microbial potential was compare with comparator products the herbal kajal was evaluated. Kohl’s have been used since antiquity in various civilizations of the world, and dates back to the Bronze Age. It is chiefly used by the females of South Asia, Middle East and Africa. It is known as “qwalli” in West Africa and “surma”, “kajal”, or “kaadige” in South Asia. The concerns are even more grave since kohl finds greater acceptability in women and infant population. Herbal kohl was formulated and scientific intent was used for selection of ingredients. The herbal kohl formulated showed antimicrobial activity, the major concern of the study

Keywords : Kohl, Kajal, Surma, Herbal Kohl.