Authors :
D.Nagavalli; P.Ponguzhali; J.Jayamathi
Volume/Issue :
Volume 8 - 2023, Issue 8 - August
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/2p853caw
DOI :
https://doi.org/10.5281/zenodo.8272944
Abstract :
A simple, exact, and reliable reverse phase
liquid chromatographic technique was developed for the
simultaneous detection of paracetamol, ambroxol
hydrochloride, levocetirizine dihydrochloride, and
pseudoephedrine hydrochloride in tablet dosage forms.
The analysis was carried out using C18 (150 mm x 4.6
mm i.d.) and a mobile phase with an acetonitrile : water
(pH6 adjusted with orthophosphoric acid) ratio of
(60:40). The detection employed a flow rate of 1.0 ml/min
and a wavelength of 257 nm. Levocetirizine
dihydrochloride, pseudoephedrine hydrochloride,
ambroxol hydrochloride, and paracetamol all had
retention periods that were, respectively, 2.396 minutes,
4.829 minutes, 2.807 minutes, and 3.620 minutes.
According to ICH recommendations, the method was
authorized. The approach's specificity, precision,
linearity, accuracy, and robustness were all confirmed.
For paracetamol, ambroxol hydrochloride, levocetirizine
dihydrochloride, and pseudoephedrine hydrochloride,
the linearity ranged from 4 to 24 g/ml.The proposed method was
successfully used to measure paracetamol, ambroxol
hydrochloride, levocetirizine dihydrochloride, and
pseudoephedrine hydrochloride in combination tablet
formulation.
Keywords :
Paracetamol, Ambroxol Hydrochloride, Levocetirizine Dihydrochloride, Pseudoephedrine Hydrochloride, RP-HPLC Method.
A simple, exact, and reliable reverse phase
liquid chromatographic technique was developed for the
simultaneous detection of paracetamol, ambroxol
hydrochloride, levocetirizine dihydrochloride, and
pseudoephedrine hydrochloride in tablet dosage forms.
The analysis was carried out using C18 (150 mm x 4.6
mm i.d.) and a mobile phase with an acetonitrile : water
(pH6 adjusted with orthophosphoric acid) ratio of
(60:40). The detection employed a flow rate of 1.0 ml/min
and a wavelength of 257 nm. Levocetirizine
dihydrochloride, pseudoephedrine hydrochloride,
ambroxol hydrochloride, and paracetamol all had
retention periods that were, respectively, 2.396 minutes,
4.829 minutes, 2.807 minutes, and 3.620 minutes.
According to ICH recommendations, the method was
authorized. The approach's specificity, precision,
linearity, accuracy, and robustness were all confirmed.
For paracetamol, ambroxol hydrochloride, levocetirizine
dihydrochloride, and pseudoephedrine hydrochloride,
the linearity ranged from 4 to 24 g/ml.The proposed method was
successfully used to measure paracetamol, ambroxol
hydrochloride, levocetirizine dihydrochloride, and
pseudoephedrine hydrochloride in combination tablet
formulation.
Keywords :
Paracetamol, Ambroxol Hydrochloride, Levocetirizine Dihydrochloride, Pseudoephedrine Hydrochloride, RP-HPLC Method.
