Authors :
Omambia M. Vincent; ORCID Nguta M. Joseph; Mitema E. Simon; Moriasi A. Gervason; ORCID and Onyancha M. Jared
Volume/Issue :
Volume 9 - 2024, Issue 3 - March
Google Scholar :
https://tinyurl.com/3r3tn8ry
Scribd :
https://tinyurl.com/2s3ttwdk
DOI :
https://doi.org/10.38124/ijisrt/IJISRT24MAR1443
Abstract :
In light of the enduring obstacles encountered
in analgesia and anti-inflammatory therapeutics despite
the strides made in contemporary medical sciences, the
quest for alternative efficacious strategies is an imperative
pursuit. Thus, this study investigated the safety profiles, as
well as the analgesic and anti-inflammatory attributes, of
aqueous and methanolic leaf extracts derived from
Hypericum revolutum subsp. keniense—a botanical entity
entrenched in historical ethnomedicinal practices in the
Kenyan context. The assessment of acute oral toxicity of
these extracts was conducted in accordance with the Up-
and-Down method advocated by the Organisation for
Economic Cooperation and Development (OECD),
utilizing Swiss albino mice as the experimental subjects.
Subsequent investigation entailed the evaluation of
antinociceptive and anti-inflammatory properties
employing the acetic acid-induced writhing and
carrageenan-induced paw oedema methodologies,
respectively, in the same murine model. The findings of
this study evince the safety of both aqueous and
methanolic extracts, with LD50 values surpassing 2000
mg/kg body weight and the absence of discernible signs of
toxicity. Furthermore, a notable dose-dependent (P<0.05)
manifestation of analgesic effects was observed for both
extracts, with the aqueous variant demonstrating
heightened potency compared to its methanolic
counterpart (P<0.05). Moreover, the anti-inflammatory
efficacy escalated significantly with escalating extract
doses and prolonged treatment duration (P<0.05), wherein
the aqueous extract showcased superior effectiveness vis-
à-vis the methanolic extract across all dosage regimens
and temporal checkpoints (P<0.05). Therefore, this
investigation underscores the latent promise of the
examined extracts as reservoirs of safe and efficacious
analgesic and anti-inflammatory agents, thereby meriting
further meticulous exploration. Subsequent research
endeavours ought to pivot towards elucidating the
phytochemical constituents underpinning the observed
effects, alongside unravelling their mechanistic
underpinnings across a spectrum of animal models and
clinical milieus.
Keywords :
Acetic Acid-Induced Writhing; Carrageenan- Induced Paw Oedema; Toxicity; Pain; Inflammation; Phytochemicals.
In light of the enduring obstacles encountered
in analgesia and anti-inflammatory therapeutics despite
the strides made in contemporary medical sciences, the
quest for alternative efficacious strategies is an imperative
pursuit. Thus, this study investigated the safety profiles, as
well as the analgesic and anti-inflammatory attributes, of
aqueous and methanolic leaf extracts derived from
Hypericum revolutum subsp. keniense—a botanical entity
entrenched in historical ethnomedicinal practices in the
Kenyan context. The assessment of acute oral toxicity of
these extracts was conducted in accordance with the Up-
and-Down method advocated by the Organisation for
Economic Cooperation and Development (OECD),
utilizing Swiss albino mice as the experimental subjects.
Subsequent investigation entailed the evaluation of
antinociceptive and anti-inflammatory properties
employing the acetic acid-induced writhing and
carrageenan-induced paw oedema methodologies,
respectively, in the same murine model. The findings of
this study evince the safety of both aqueous and
methanolic extracts, with LD50 values surpassing 2000
mg/kg body weight and the absence of discernible signs of
toxicity. Furthermore, a notable dose-dependent (P<0.05)
manifestation of analgesic effects was observed for both
extracts, with the aqueous variant demonstrating
heightened potency compared to its methanolic
counterpart (P<0.05). Moreover, the anti-inflammatory
efficacy escalated significantly with escalating extract
doses and prolonged treatment duration (P<0.05), wherein
the aqueous extract showcased superior effectiveness vis-
à-vis the methanolic extract across all dosage regimens
and temporal checkpoints (P<0.05). Therefore, this
investigation underscores the latent promise of the
examined extracts as reservoirs of safe and efficacious
analgesic and anti-inflammatory agents, thereby meriting
further meticulous exploration. Subsequent research
endeavours ought to pivot towards elucidating the
phytochemical constituents underpinning the observed
effects, alongside unravelling their mechanistic
underpinnings across a spectrum of animal models and
clinical milieus.
Keywords :
Acetic Acid-Induced Writhing; Carrageenan- Induced Paw Oedema; Toxicity; Pain; Inflammation; Phytochemicals.
