Authors : Nilesh Lavhate, Mukul Barwant

Volume/Issue : Volume 5 - 2020, Issue 4 - April

Google Scholar : https://goo.gl/DF9R4u

Scribd : https://bit.ly/3bNW7YJ

Abstract : Agricultural production is depends on qualitative and quantitative of yeids . A productivity of agricultural plant is decide by the crop quality mostly depend on seed. If seed abnormal ,inferior quality it means the less productivity . Such inferior quality should be induce due to infection of seed borne pathogen result in seed rot, seedling decay and abnormalities, discolorations , seed size will be shorter and shriveled of seed.These symptom occurs when pathogen growing that causing the diseases ,when any diseased they shows such symptoms which reduced the productivity of crop .So that we have to study different types of pathogen . To study such pathogen and their widespread and effect crop . First we had to collection of plant material which infected by the pathogen and from pathogen we have to made culture then it should to be grown to be nutrient medium . The collection of plant material is maize and soybean seed .From that we identify which is pathogen by its morphological and reproductiove structure . On the basis of structure of conidia we identify the given pathogen are Alternaria solani and Fusarium oxysporum.They are isolated from plant material then culture are made they are grown on the nutrient media (potato dextrose agar ) Then growth will occurred that preserved and maintained for different purpose.

Keywords : Alternaria solani and Fusarium oxysporum, Collection of material, isolation and maintainance.