Authors : Leleji Jeffree; Timothy Adejoh Attah; Abubakar Adamu; John-Paul Ojochenemi Enemali; Olayinka Oyedola; Elizabeth Aregbe; Mariya Akilu; Bright Evabor; Zainab Ibrahim Suleiman; Ejura Nana Abu; Ishaya Daniel Bwala; Sofia Suleiman Hussein; Aliya Ademu; Fadimatu Sanusi; Farida Mohammed Shehu; Chinwe Vivian Igwe; Polok Caleb; Ifeanyichukwu Ezeh

Volume/Issue : Volume 11 - 2026, Issue 3 - March

Google Scholar : https://tinyurl.com/7td25vsc

Scribd : https://tinyurl.com/5cwf7bkw

DOI : https://doi.org/10.38124/ijisrt/26mar856

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Background: It is important to understand plant responses to altered gravity as it is important to advance space agriculture and improve food sustsainability. The effect of microgravity may elicit significant physiologic and morphologic changes that can be important to the economic growth of soya bean seedlings. This study assessed the morphological effects of simulated microgravity on soya bean seedling.  Method: Soya bean seedlings was germinated on agar medium on a petri dish and were assigned to horizontal, 90 ° orientation and clinorotation groups. Root growth, length and curvature were monitored after 24 and 48 hours. Data was collected and analyzed using SPSS  Results: After 24 hours, root length of clinorotated (0.75cm) seedlings was higher compared to those vertically (0.58cm) and horizontally (0.50cm) controls after 24 hours (P<.05). However, vertical group (2.52cm) had the highest root growth after 48 hours (P<.05). The root curvature was higher in the clinorotated (27.11ᵒ) and horizontally (29.75ᵒ) placed seedlings after 24 hours. After 48 hours, the vertically (33.41ᵒ) and horizontally (40.51ᵒ) placed seedlings had higher curvature compared to the clinorotated (26.14) group but this was not statistically significant (P>.05).  Conclusion: This study finding reveals that short-term clinorotation can enhance early root growth. The study elucidates early plant development with altered gravity and provides insight necessary for space biology and improving agriculture.

Keywords : Soya Bean, Microgravity, Clinostat, Clinorotation, Glycine Max.

References :

1. Hassanpour H, Abdel Latef AAH. Changes in chlorophyll metabolism, Rubisco gene expression, and antioxidant metabolites of Ocimum basilicum seedlings subjected to clinorotation. Plant Cell Tissue Organ Cult PCTOC 2023;153:119–32. https://doi.org/10.1007/s11240-023-02448-z.
2. NSF. Gravity on Earth n.d. https://nsf-gov-resources.nsf.gov/files/Gravity.pdf.
3. Farooq M, Ali S, Khan M, Jang Y-H, Kim E-G, Zhao D-D, et al. Investigating plant responses to microgravity and adaptations in gravisensitive environments. Environ Sci Eur 2024;36:28. https://doi.org/10.1186/s12302-024-00851-7.
4. Villacampa A, Fañanás‐Pueyo I, Medina FJ, Ciska M. Root growth direction in simulated microgravity is modulated by a light avoidance mechanism mediated by flavonols. Physiol Plant 2022;174:e13722. https://doi.org/10.1111/ppl.13722.
5. Ambiya R, R B, M.s N. Exploring Microgravity’s Effect on Seed Germination and Growth of Plants. Int J Plant Soil Sci 2024;36:75–90. https://doi.org/10.9734/ijpss/2024/v36i125185.
6. Wang X, Komatsu S. Improvement of Soybean Products Through the Response Mechanism Analysis Using Proteomic Technique. Adv. Food Nutr. Res., vol. 82, Elsevier; 2017, p. 117–48. https://doi.org/10.1016/bs.afnr.2016.12.006.
7. Wang X, Komatsu S. Proteomic approaches to uncover the flooding and drought stress response mechanisms in soybean. J Proteomics 2018;172:201–15. https://doi.org/10.1016/j.jprot.2017.11.006.
8. Messina M. Perspective: Soybeans Can Help Address the Caloric and Protein Needs of a Growing Global Population. Front Nutr 2022;9:909464. https://doi.org/10.3389/fnut.2022.909464.
9. Vargas-Almendra A, Ruiz-Medrano R, Núñez-Muñoz LA, Ramírez-Pool JA, Calderón-Pérez B, Xoconostle-Cázares B. Advances in Soybean Genetic Improvement. Plants 2024;13. https://doi.org/10.3390/plants13213073.
10. Effect of Six Decades of Selective Breeding on Soybean Protein Composition and Quality: A Biochemical and Molecular Analysis. ACS Publ 2006. https://pubs.acs.org/doi/abs/10.1021/jf060391m (accessed February 3, 2026).
11. United Nations. Programme on Space Application Teacher’s Guide to Plant Experiments in Microgravity. New York: 2013.
12. Hilaire E, Peterson BV, Guikema JA, Brown CS. Clinorotation affects morphology and ethylene production in soybean seedlings. Plant Cell Physiol 1996;37:929–34. https://doi.org/10.1093/oxfordjournals.pcp.a029041.
13. Miyamoto K, Oka M, Yamamoto R, Masuda Y, Hoson T, Kamisaka S, et al. Auxin polar transport in Arabidopsis under simulated microgravity conditions — Relevance to growth and development. Adv Space Res 1999;23:2033–6. https://doi.org/10.1016/S0273-1177(99)00344-0.
14. Gao J, Zhuang S, Zhang W. Advances in Plant Auxin Biology: Synthesis, Metabolism, Signaling, Interaction with Other Hormones, and Roles under Abiotic Stress. Plants 2024;13:2523. https://doi.org/10.3390/plants13172523.
15. Paul A-L, Amalfitano CE, Ferl RJ. Plant growth strategies are remodeled by spaceflight. BMC Plant Biol 2012;12:232. https://doi.org/10.1186/1471-2229-12-232.
16. Medina FJ, Herranz R. Microgravity environment uncouples cell growth and cell proliferation in root meristematic cells: The mediator role of auxin. Plant Signal Behav 2010;5:176–9. https://doi.org/10.4161/psb.5.2.10966.
17. Du J, Zeng L, Yu Z, Chen S, Chen X, Zhang Y, et al. A magnetically enabled simulation of microgravity represses the auxin response during early seed germination on a microfluidic platform. Microsyst Nanoeng 2022;8:11. https://doi.org/10.1038/s41378-021-00331-5.
18. Masson PH, Tasaka M, Morita MT, Guan C, Chen R, Boonsirichai K. Arabidopsis thaliana: A Model for the Study of Root and Shoot Gravitropism. Arab Book Am Soc Plant Biol 2002;1:e0043. https://doi.org/10.1199/tab.0043.
19. Aluko OE, Onabowale MK. Effect of Gravity Variation on the Growth of Wheat and Guinea Corn Seedlings. J Exp Agric Int 2019:1–9. https://doi.org/10.9734/JEAI/2019/31325.
20. Oluwafemi F. Gravity Variation Effects on the Growth of Maize Shoots. Phys Sci Forum 2021;2. https://doi.org/10.3390/ECU2021-10184.
21. Mullen JL, Wolverton C, Ishikawa H, Evans ML. Kinetics of Constant Gravitropic Stimulus Responses in Arabidopsis Roots Using a Feedback System. Plant Physiol 2000;123:665–70. https://doi.org/10.1104/pp.123.2.665.