Authors :
D.NAGAVALLI; R.SHANMUGAVEL
Volume/Issue :
Volume 8 - 2023, Issue 2 - February
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3ICcPx6
DOI :
https://doi.org/10.5281/zenodo.7659258
Abstract :
Three simple, precise, accurate, specific and
reproducible spectrophotometric methods, requiring no
prior separation, have been developed for the
simultaneous estimation of atrovastatin calcium (ATV)
and fenofibrate (FEN) in combined dosage form.
Quantitative estimation of these drugs in marketed
brands of the tablet was carried out using simultaneous
equation, absorbance ratio and first order derivative
methods. In the simultaneous equation method ATV and
FEN have absorbance maxima at 247 and 289 nm
respectively in methanol. Second method employs Q
absorbance analysis using 254 nm (Isobestic point) and
289 nm ( max of FEN) as two wavelengths for
estimation. The Third method involves First order
derivative spectroscopy using 236 nm and 246 nm as
zero crossing points for ATV and FEN respectively.
Linearity was observed in the concentration range of 0-
24 µg/ml for both ATV and FEN. Accuracy and
Precision of the method was determined and validated
statistically. Method showed good reproducibility and
recovery with % RSD less than 2%. The methods were
successfully applied for determining the amount in
marketed formulation.
Keywords :
Atrovastatin Calcium, Fenofibrate, Simultaneous Equation, Absorbance Ratio, First Order Derivative.
Three simple, precise, accurate, specific and
reproducible spectrophotometric methods, requiring no
prior separation, have been developed for the
simultaneous estimation of atrovastatin calcium (ATV)
and fenofibrate (FEN) in combined dosage form.
Quantitative estimation of these drugs in marketed
brands of the tablet was carried out using simultaneous
equation, absorbance ratio and first order derivative
methods. In the simultaneous equation method ATV and
FEN have absorbance maxima at 247 and 289 nm
respectively in methanol. Second method employs Q
absorbance analysis using 254 nm (Isobestic point) and
289 nm ( max of FEN) as two wavelengths for
estimation. The Third method involves First order
derivative spectroscopy using 236 nm and 246 nm as
zero crossing points for ATV and FEN respectively.
Linearity was observed in the concentration range of 0-
24 µg/ml for both ATV and FEN. Accuracy and
Precision of the method was determined and validated
statistically. Method showed good reproducibility and
recovery with % RSD less than 2%. The methods were
successfully applied for determining the amount in
marketed formulation.
Keywords :
Atrovastatin Calcium, Fenofibrate, Simultaneous Equation, Absorbance Ratio, First Order Derivative.
