Simultaneous Equation, Absorbance Ratio and First Order Derivative Methods for the Estimation of Atorvastatin Calcium and Fenofibrate in Solid Dosage Form


Authors : D.NAGAVALLI; R.SHANMUGAVEL

Volume/Issue : Volume 8 - 2023, Issue 2 - February

Google Scholar : https://bit.ly/3IIfn9N

Scribd : https://bit.ly/3ICcPx6

DOI : https://doi.org/10.5281/zenodo.7659258

Abstract : Three simple, precise, accurate, specific and reproducible spectrophotometric methods, requiring no prior separation, have been developed for the simultaneous estimation of atrovastatin calcium (ATV) and fenofibrate (FEN) in combined dosage form. Quantitative estimation of these drugs in marketed brands of the tablet was carried out using simultaneous equation, absorbance ratio and first order derivative methods. In the simultaneous equation method ATV and FEN have absorbance maxima at 247 and 289 nm respectively in methanol. Second method employs Q absorbance analysis using 254 nm (Isobestic point) and 289 nm ( max of FEN) as two wavelengths for estimation. The Third method involves First order derivative spectroscopy using 236 nm and 246 nm as zero crossing points for ATV and FEN respectively. Linearity was observed in the concentration range of 0- 24 µg/ml for both ATV and FEN. Accuracy and Precision of the method was determined and validated statistically. Method showed good reproducibility and recovery with % RSD less than 2%. The methods were successfully applied for determining the amount in marketed formulation.

Keywords : Atrovastatin Calcium, Fenofibrate, Simultaneous Equation, Absorbance Ratio, First Order Derivative.

Three simple, precise, accurate, specific and reproducible spectrophotometric methods, requiring no prior separation, have been developed for the simultaneous estimation of atrovastatin calcium (ATV) and fenofibrate (FEN) in combined dosage form. Quantitative estimation of these drugs in marketed brands of the tablet was carried out using simultaneous equation, absorbance ratio and first order derivative methods. In the simultaneous equation method ATV and FEN have absorbance maxima at 247 and 289 nm respectively in methanol. Second method employs Q absorbance analysis using 254 nm (Isobestic point) and 289 nm ( max of FEN) as two wavelengths for estimation. The Third method involves First order derivative spectroscopy using 236 nm and 246 nm as zero crossing points for ATV and FEN respectively. Linearity was observed in the concentration range of 0- 24 µg/ml for both ATV and FEN. Accuracy and Precision of the method was determined and validated statistically. Method showed good reproducibility and recovery with % RSD less than 2%. The methods were successfully applied for determining the amount in marketed formulation.

Keywords : Atrovastatin Calcium, Fenofibrate, Simultaneous Equation, Absorbance Ratio, First Order Derivative.

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe