Authors :
Kyado Aondohemba
Volume/Issue :
Volume 8 - 2023, Issue 4 - April
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/44yyp2kc
DOI :
https://doi.org/10.5281/zenodo.8281031
Abstract :
The discharge of spent automobile oil from
automobile, industrial machines, plants and electric
power generators are great concern in automobile
workshop as they littered and contaminate soil. Spent
automobile oil degradation potentials of bacteria isolates
from soil in mechanic workshops within Makurdi was
carried out. Eighty (80) samples comprising of 40 top soil
and 40 sub soil were collected from the four mechanic
workhouses in Makurdi; (Apir, Wurukum, North bank
and New Garage). The samples were pour plated on
mineral salt agar from where total viable counts was
done and discrete colonies sub-cultured to obtain pure
isolates. Fresh automobile spent oil was inoculated with
bacteria isolates and incubated for five days to
determine their biodegradation. Optical density of the
spent automobile oil was determined in Aucm-1
at 600nm
for every 24 hours. Bacteria specie found to possess
biodegradation potentials were subjected to molecular
analysis for full identification.
The discharge of spent automobile oil from
automobile, industrial machines, plants and electric
power generators are great concern in automobile
workshop as they littered and contaminate soil. Spent
automobile oil degradation potentials of bacteria isolates
from soil in mechanic workshops within Makurdi was
carried out. Eighty (80) samples comprising of 40 top soil
and 40 sub soil were collected from the four mechanic
workhouses in Makurdi; (Apir, Wurukum, North bank
and New Garage). The samples were pour plated on
mineral salt agar from where total viable counts was
done and discrete colonies sub-cultured to obtain pure
isolates. Fresh automobile spent oil was inoculated with
bacteria isolates and incubated for five days to
determine their biodegradation. Optical density of the
spent automobile oil was determined in Aucm-1
at 600nm
for every 24 hours. Bacteria specie found to possess
biodegradation potentials were subjected to molecular
analysis for full identification.
