Authors : Gayathri K.; Syam K. Venugopal; Anoop S.; John Martin K.D.; Lucy K.M.; Shynu M.

Volume/Issue : Volume 10 - 2025, Issue 8 - August

Google Scholar : https://tinyurl.com/3anmpzzc

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DOI : https://doi.org/10.38124/ijisrt/25aug186

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Abstract : This pilot study evaluated the efficacy of three commonly prescribed topical steroid eye drops in inducing a minimally invasive, short-term ocular hypertensive (OHT) glaucoma model in New Zealand White rabbits. The agents tested included commercial betamethasone, dexamethasone and prednisolone eyedrops solutions. Their effectiveness was assessed based on the consistency of intraocular pressure (IOP) elevation and the presence of clinically significant side effects. IOP was measured daily using both Tonopen and Schiotz tonometers following topical analgesia. The progression of IOP over a three-week period was recorded and analyzed to determine the suitability of each steroid for inducing short-term OHT. Prednisolone administration resulted in marked fluctuations in body condition scores, protein loss, and body weight, although no mortality was observed. Dexamethasone induced higher IOP spikes but lacked consistency. In contrast, betamethasone produced a sustained and consistent elevation in IOP without notable adverse effects, making it the most promising candidate for short-term glaucoma modelling. This minimally invasive model provides a reliable platform for short-term glaucoma research, with strong potential for translational applications in diagnostics and therapeutic evaluation.

Keywords : Steroid, Eyedrops, Ocular Hypertension, Glaucoma Model, Rabbits, Intraocular Pressure.

References :

1. W.K. Abdulsahib and S.J. Abood, “The effect of calcium channel blocker in the betamethasone induced glaucoma model in rabbits,” J. Adv. Pharm. Educ. Res., vol. 11, no. 1, pp. 135–140, 2021.
2. L. Bonomi, S. Perfetti, R. Bellucci, F. Massa, and E. Noya, “Ocular hypotensive action of labetalol in rabbit and human eyes,” Albrecht von Graefes Arch. Klin. Ophthalmol., vol. 217, pp. 175–181, 1981.
3. J.C. Fleischhauer, R. Liu, P. Elena, J. Flammer, and I.O. Haefliger, “Title not provided,” Klin. Monatsbl. Augenheilkd., vol. 218, pp. 351–353, 2001.
4. S.D. Gupta, J.S. Gupta, S.B. Gupta, and B.G. Mahajan, “Steroid induced glaucoma (experimental study),” Indian J. Ophthalmol., vol. 17, pp. 14–16, 1969.
5. B. Hou, F. Wang, Z. Ye, X. Jin, Y. Fu, and Z. Liu, “Study of minimally invasive radiofrequency ablation of ciliary body for the treatment of glaucoma in rabbits,” Mol. Med. Rep., vol. 21, pp. 1071–1076, 2019.
6. Y. Qin, S. Lam, G.H.F. Yam, K.W. Choy, D.T.L. Liu, T.Y.H. Chiu, W.Y. Li, D.S.C. Lam, C.P. Pang, and D.S.P. Fan, “A rabbit model of age dependent ocular hypertensive response to topical corticosteroids,” Acta Ophthalmol., pp. 1–5, 2012.
7. D. Lu, C. Chang, and J. Wu, “The changes of vitreous pH values in an acute glaucoma rabbit model,” J. Ocul. Pharmacol. Ther., vol. 17, pp. 343–350, 2001.
8. W.F. March, T. Gherezghiher, M. Koss, and R. Nordquist, “Ultrastructural and pharmacologic studies on laser-induced glaucoma in primates and rabbits,” Lasers Surg. Med., vol. 4, pp. 329–335, 1984.
9. A.A. ElGohary and L.H.M. Elshazly, “Photopic negative response in diagnosis of glaucoma: an experimental study in glaucomatous rabbit model,” Int. J. Ophthalmol., vol. 8, no. 3, pp. 459–464, 2015.
10. N.M. Muharib and H.A. Al-Timmemi, “Trypsin dose optimisation for glaucoma induction in rabbit model,” Iraqi J. Vet. Sci., vol. 35, pp. 107–113, 2021.
11. M. Orihashi, Y. Shima, H. Tsuneki, and I. Kimura, “Potent reduction of intraocular pressure by nilpradilol plus latanoprost in ocular hypertensive rabbits,” Biol. Pharm. Bull., vol. 28, no. 1, pp. 65–68, 2005.
12. S. Panchal, A.A. Mehta, and D.D. Santani, “Effect of potassium channel openers in acute and chronic models of glaucoma,” Taiwan J. Ophthalmol., vol. 6, pp. 131–135, 2016.
13. F.Q. Pereira, B.S. Bercht, M.G. Soares, M.G.B. da Mota, and J.A.T. Pigatto, “Comparison of a rebound and an applanation tonometer for measuring intraocular pressure in normal rabbits,” Vet. Ophthalmol., vol. 14, no. 5, pp. 321–326, 2011.
14. W.M.S. Russell and R.L. Burch, *The principles of humane experimental technique*, 1st ed. London: Methuen, 1959, pp. 238.
15. R.M. Sappington, B.J. Carlson, S.D. Crish, and D.J. Calkins, “The microbead occlusion model: a paradigm for induced ocular hypertension in rats and mice,” Invest. Ophthalmol. Vis. Sci., vol. 51, pp. 207–216, 2010.
16. T. Stahnke, S.E. Walther, W. Schimdt, O. Stachs, K.P. Schimtz, and R.F. Guthoff, “Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model,” Curr. Dir. Biomed. Eng., vol. 1, pp. 127–130, 2015.
17. Z. Song, Y. Gong, H. Liu, Q. Ren, and X. Sun, “Glycyrrhizin could reduce ocular hypertension induced by triamcinolone acetonide in rabbits,” Mol. Vis., vol. 17, pp. 2056–2064, 2011.
18. Wang, Q., Wang, J., Lin, X., Lui, K.H., Chang, S., Zhou, R. and Lam, D.S. 2019. Inducing chronic ocular hypertension in rabbits via limbal buckling. Br. J. Ophthalmol. 103: 144–151.
19. D. Yuschenkoff, J. Graham, and S.A. Pumphrey, “Diagnosis and treatment of glaucoma in client-owned rabbits (Oryctolagus cuniculus): 16 eyes from 11 rabbits (2008–2019),” J. Exot. Pet Med., pp. 1–22, 2020.
20. E. Zernii, V.E. Baksheeva, E.N. Iomdina, O.A. Averina, S.E. Permyakov, P.P. Philippov, A.A. Zamyatnin Jr, and I.I. Senin, “Rabbit models of ocular diseases: new relevance for classical approaches,” CNS Neurol. Disord.-Drug Targets, vol. 15, no. 1, pp. 1–25, 2016.