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Study of Bacterial Strain Isolated from Pigeon Pea Rhizosphere and their Role as Biofertilizers


Authors : Louas Chikara; Sachin Nagar; Ayush Saini

Volume/Issue : Volume 11 - 2026, Issue 4 - April


Google Scholar : https://tinyurl.com/4bxdnfsn

Scribd : https://tinyurl.com/37f22x3c

DOI : https://doi.org/10.38124/ijisrt/26apr2091

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : The transition toward sustainable farming necessitates a reduction in chemical fertilizer dependency through the use of biologically active alternatives. Rigorous and rigid contraction can increase the quantity of chemical fertilizers that will deplete the soil because of decreasing the penetration capacity of water into the soil. This paper outlines a comprehensive framework for the isolation of potent nitrogen-fixing bacteria, specifically Pseudomonas, Azotobacter, and Rhizobium, from the rhizosphere soil of the pigeon pea plant at MIET, Meerut, Uttar Pradesh Morphological and biochemical tests were performed after the successful isolation of the bacterial strains in which all the bacterial strains were found gram-negative bacteria under morphological test. Catalase, MR-VP, Carbohydrate fermentation, urea and indole tests were also done for biochemical characterization. These isolated strains are proposed to be formulated into a multi-strain biofertilizer consortium designed for application on ornamental flowering plants. In the present study, we have conducted test trials in pots for observing the effect of isolated bacterial strains on flowering plants, and all the three bacterial strains were cultured in the Urea broth. The broth was applied as Seed Treatment, soil application by enriching compost at the time of sowing and later by drenching in the flowering plants. As a result, the proposed approach aims to enhance soil fertility, balanced pH, EC, increased OC and N, early blooming of flowers, improve plant growth metrics, and promote sustainable agricultural practices. This study provides a structured methodology and evaluation plan to validate the efficacy of rhizosphere bacterial consortiums in horticultural applications.

Keywords : Sustainable, Penetration, Biofertilizers, Ornamental, Horticultural.

References :

  1. Gupta, S., Didwania, N., Srinivasa, N. (2020). Role of Biofertilizer in Biological Management of Fungal Diseases of Pigeon Pea [(Cajanus cajan) (L.) Millsp.]. In: Singh, B., Singh, G., Kumar, K., Nayak, S., Srinivasa, N. (eds) Management of Fungal Pathogens in Pulses. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-35947-8_12.
  2. Arora, N.K., Khare, E., Singh, S. et al. Phenetic, genetic diversity and symbiotic compatibility of rhizobial strains nodulating pigeon pea in Northern India. 3 Biotech 8, 52 (2018). https://doi.org/10.1007/s13205-017-1074-1.
  3. Aktar, M. W., Sengupta, D., & Chowdhury, A. (2009). Impact of pesticides use in agriculture: their benefits and hazards. Interdisciplinary toxicology2(1), 1.
  4. Verma, P. and Shahi, S.K., 2015. Characterization of plant growth promoting rhizobacteria associated with potato rhizosphere. Int J Adv Res3(6), pp.564-572.
  5. Saharan, B. S., & Nehra, V. (2011). Plant growth promoting rhizobacteria: a critical review. Life Sci Med Res21(1), 30.
  6. Kloepper, Joseph W. "Plant growth-promoting rhizobacteria on radishes." In Proc. of the 4th Internet. Conf. on Plant Pathogenic Bacter, Station de Pathologie Vegetale et Phytobacteriologie, INRA, Angers, France, 1978, vol. 2, pp. 879-882. 1978.
  7. Sun, W., Shahrajabian, M. H., & Soleymani, A. (2024). The Roles of Plant-Growth-Promoting Rhizobacteria (PGPR)-Based Biostimulants for Agricultural Production Systems. Plants13(5), 613. https://doi.org/10.3390/plants13050613.
  8. Teja, A.R., Leona, G., Prasanth, J., Yatung, T., Singh, S. and Bhargav, V., 2023. Role of plant growth–promoting rhizobacteria in sustainable agriculture. In Advanced Microbial Technology for Sustainable Agriculture and Environment (pp. 175-197). Academic Press. https://doi.org/10.1016/B978-0-323-95090-9.00001-7.
  9. Ahmad, Farah, Iqbal Ahmad, and M. S. Khan. "Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities." Microbiological research 163.2 (2008): 173-181. https://doi.org/10.1016/j.micres.2006.04.001.
  10. Adesemoye, A.O., Kloepper, J.W. Plant–microbes interactions in enhanced fertilizer-use efficiency. Appl Microbiol Biotechnol 85, 1–12 (2009). https://doi.org/10.1007/s00253-009-2196-0.
  11. Banerjee M.R., Yesmin L., Vessey, J.K. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil 255, 571–586 (2003). https://doi.org/10.1023/A:1026037216893.

The transition toward sustainable farming necessitates a reduction in chemical fertilizer dependency through the use of biologically active alternatives. Rigorous and rigid contraction can increase the quantity of chemical fertilizers that will deplete the soil because of decreasing the penetration capacity of water into the soil. This paper outlines a comprehensive framework for the isolation of potent nitrogen-fixing bacteria, specifically Pseudomonas, Azotobacter, and Rhizobium, from the rhizosphere soil of the pigeon pea plant at MIET, Meerut, Uttar Pradesh Morphological and biochemical tests were performed after the successful isolation of the bacterial strains in which all the bacterial strains were found gram-negative bacteria under morphological test. Catalase, MR-VP, Carbohydrate fermentation, urea and indole tests were also done for biochemical characterization. These isolated strains are proposed to be formulated into a multi-strain biofertilizer consortium designed for application on ornamental flowering plants. In the present study, we have conducted test trials in pots for observing the effect of isolated bacterial strains on flowering plants, and all the three bacterial strains were cultured in the Urea broth. The broth was applied as Seed Treatment, soil application by enriching compost at the time of sowing and later by drenching in the flowering plants. As a result, the proposed approach aims to enhance soil fertility, balanced pH, EC, increased OC and N, early blooming of flowers, improve plant growth metrics, and promote sustainable agricultural practices. This study provides a structured methodology and evaluation plan to validate the efficacy of rhizosphere bacterial consortiums in horticultural applications.

Keywords : Sustainable, Penetration, Biofertilizers, Ornamental, Horticultural.

Paper Submission Last Date
31 - May - 2026

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