Study of the Effect of Dilution on the Quality of Milt of Clarias gariepinus Broodstock in Artificial Reproduction in Mali


Authors : Siaka Dembele; Diakaridia Traore; Karamogo Fane; Mahamadou Kelepily; Aboubacar Kane

Volume/Issue : Volume 10 - 2025, Issue 4 - April


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DOI : https://doi.org/10.38124/ijisrt/25apr2362

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Abstract : Clarias gariepinus is one of the species of farmed fish that has been studied and popularized in Mali. The difficulties of his reproduction in captivity have led researchers to conduct studies on the effects of thinners on the quality of his milt. The objective of this study is to test the effectiveness of Clarias gariepinus seed mobility from diluents in Mali. It was conducted on 3 batches of 9 males and 12 females from the fish farm of Bathily in Sikoulou. The average weights in each batch were respectively 846.25±2.35 (batch 1); 849.22±1.22 (batch 2); 861.40±2.68 (batch 3) male, and respectively 1190.23±53.44 (batch 1); 1167.33 ±34.15 (batch 2) and 1200.18±40.81 (batch 3) for female. The results on the volumes of milt collected gave values of 0.17g, 0.7g and 0.9g. The sperm concentration was the same for all beaches and the motility durations varied respectively during 12h. The value of pH varied between 6.73±0.06 and 6.75±0.04. All the sperm characteristics revealed a good quality of the seeds of the Clarias gariepinus strain from the farm of Bathily. Only physiological serums and drilling waters allowed fertilization after activation of the spermatozoa by the diluents. However, the results obtained with these diluents after 12 hours of storage suggest a possibility of improving their effectiveness for the artificial reproduction of Clarias gariepinus.

Keywords : Broodstock; Clarias gariepinus; Fish Milt; Diluent; Artificial Reproduction.

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Clarias gariepinus is one of the species of farmed fish that has been studied and popularized in Mali. The difficulties of his reproduction in captivity have led researchers to conduct studies on the effects of thinners on the quality of his milt. The objective of this study is to test the effectiveness of Clarias gariepinus seed mobility from diluents in Mali. It was conducted on 3 batches of 9 males and 12 females from the fish farm of Bathily in Sikoulou. The average weights in each batch were respectively 846.25±2.35 (batch 1); 849.22±1.22 (batch 2); 861.40±2.68 (batch 3) male, and respectively 1190.23±53.44 (batch 1); 1167.33 ±34.15 (batch 2) and 1200.18±40.81 (batch 3) for female. The results on the volumes of milt collected gave values of 0.17g, 0.7g and 0.9g. The sperm concentration was the same for all beaches and the motility durations varied respectively during 12h. The value of pH varied between 6.73±0.06 and 6.75±0.04. All the sperm characteristics revealed a good quality of the seeds of the Clarias gariepinus strain from the farm of Bathily. Only physiological serums and drilling waters allowed fertilization after activation of the spermatozoa by the diluents. However, the results obtained with these diluents after 12 hours of storage suggest a possibility of improving their effectiveness for the artificial reproduction of Clarias gariepinus.

Keywords : Broodstock; Clarias gariepinus; Fish Milt; Diluent; Artificial Reproduction.

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