Authors :
Ogbuka, Chibuisi Francis; Nwachukwu, Ifeanyi; Ozougwu, Jevas Chibuike; Azunna, Uchenna; Ogwo, Elisha Uko; Onuigbo, Martin Chinaecherem
Volume/Issue :
Volume 9 - 2024, Issue 1 - January
Google Scholar :
http://tinyurl.com/2bny98b5
Scribd :
http://tinyurl.com/bdzewbkv
DOI :
https://doi.org/10.5281/zenodo.10618828
Abstract :
The study was on molecular characterization
and plasmid resistance genes of pathogenic bacteria
isolated from the ear of school children. Ethical
clearance was obtained and the consent of the Pupils and
their Parents were sort for their inclusion in this study.
The specimens were obtained from pupils aged 2 to 15
years. Aural swab was collected from pre-screened
Pupils that complained of itching of the ear and had not
taken any antibiotics prior to the specimen collection.
The study area was Nkpa in Bende, Abia State. The
Bacteria isolates were characterized using 16Subunit
ribosomal Ribonucleic acid gene sequencing. at DNA
laboratory Kaduna. The isolated Bacteria were), 1.0%
Comamonas aquatic, 4.0% Stenotrophomonas
maltophilia, 5.50% Delftia acidovorans, 5.5%
Pseudomonas aeruginosa, 8.0% Achromobacter
xylosoxidans. The isolates were subjected to ascertain
plasmid genes. From the 200 sampled population,
plasmid resistant genes were isolated in 48 bacteria
isolates. Of the statistical analysis done, 14.0% isolates
were from males while the females children had 10.0%.
Effect of age on the ear infection revealed that pupils of 2
to 3 of age had the major infection of 16 (8.00%), pupils
of 4 to7 of age and 8 to 11 years had similar number of
13 (6.50%) infection while the least infected were pupils
aged 12-15 years with 6 (3.00%). Plasmid resistance
genes were identified from all the bacteria isolates, this
result reveals the presence of resistant bacteria strains.
Thus, going by this research, it is ideal to always carry
out a surveillance on the bacteria ear infection and
plasmid gene resistant before administering antibiotics
among the pupils of this locality.
Keywords :
Pathogens, Aural Swabs, Bacteria Plasmid Genes, Bacteria,16 Subunit Ribosomal Ribonucleic Acid, Inflammation, Ear Infection, Isolates, Pathogens,
The study was on molecular characterization
and plasmid resistance genes of pathogenic bacteria
isolated from the ear of school children. Ethical
clearance was obtained and the consent of the Pupils and
their Parents were sort for their inclusion in this study.
The specimens were obtained from pupils aged 2 to 15
years. Aural swab was collected from pre-screened
Pupils that complained of itching of the ear and had not
taken any antibiotics prior to the specimen collection.
The study area was Nkpa in Bende, Abia State. The
Bacteria isolates were characterized using 16Subunit
ribosomal Ribonucleic acid gene sequencing. at DNA
laboratory Kaduna. The isolated Bacteria were), 1.0%
Comamonas aquatic, 4.0% Stenotrophomonas
maltophilia, 5.50% Delftia acidovorans, 5.5%
Pseudomonas aeruginosa, 8.0% Achromobacter
xylosoxidans. The isolates were subjected to ascertain
plasmid genes. From the 200 sampled population,
plasmid resistant genes were isolated in 48 bacteria
isolates. Of the statistical analysis done, 14.0% isolates
were from males while the females children had 10.0%.
Effect of age on the ear infection revealed that pupils of 2
to 3 of age had the major infection of 16 (8.00%), pupils
of 4 to7 of age and 8 to 11 years had similar number of
13 (6.50%) infection while the least infected were pupils
aged 12-15 years with 6 (3.00%). Plasmid resistance
genes were identified from all the bacteria isolates, this
result reveals the presence of resistant bacteria strains.
Thus, going by this research, it is ideal to always carry
out a surveillance on the bacteria ear infection and
plasmid gene resistant before administering antibiotics
among the pupils of this locality.
Keywords :
Pathogens, Aural Swabs, Bacteria Plasmid Genes, Bacteria,16 Subunit Ribosomal Ribonucleic Acid, Inflammation, Ear Infection, Isolates, Pathogens,
