Authors :
Thakir U. Mohsen
Volume/Issue :
Volume 11 - 2026, Issue 7 - July
Google Scholar :
https://tinyurl.com/49y7er3a
Scribd :
https://tinyurl.com/bde2aep3
DOI :
https://doi.org/10.38124/ijisrt/26jul240
Note : A published paper may take 4-5
working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and
ResearchGate.
Abstract :
his study investigated the association between single-nucleotide polymorphisms (SNPs) in the cysteine- and
glycine-rich protein 2 gene (CSRP2) and body dimensions in Awassi sheep. Blood samples and measurements of body length,
front height, and back height were collected from 50 animals raised at a private station in Babylon Province, Iraq, during
2022–2023. Genomic DNA was extracted, and exon 7, together with the adjacent terminal region, was amplified and
sequenced. Allele and genotype frequencies were assessed using the chi-square test, while genotype effects were analyzed
with a general linear model followed by Duncan’s multiple range test. Two C>T substitutions were identified at positions
20315 and 20094, producing the CC, CT, and TT genotypes. The T allele was the most frequent at both positions, with
frequencies of 0.67 at position 20315 and 0.57 at position 20094.
Keywords :
Awassi Sheep; CSRP2 Gene; Single Nucleotide Polymorphism; Body Dimensions; Growth Traits; Marker-Assisted Selection.
References :
- Abayomi S. Faponle, Sam P. de Visser. 2017. Advances in Inorganic Chemistry, Short-Lived Iron-Dioxygen Complex in Cysteine Dioxygenase Enzymes.
- Brants J., Kostyantyn Semenchenko1, Christine Wasylyk1, Aude Robert2, Annaick Carles. Tubulin Tyrosine Ligase Like 12, a TTLL Family Member with SET- and TTL-Like Domains and Roles in Histone and Tubulin Modifications and Mitosis. PLOS ONE | www.plosone.org.
- Cao, Y.; Y. Ai; X. Zhang; J. Zhang; X. Long; Y. Zhu; L. Wang; Q. Gu and H. Han . 2023. Genome-wide epigenetic dynamics during postnatal skeletal muscle growth in Hu sheep. https://doi.org/ 10.1038 /s4 2003-023-05439-0.
- Duncan, D.B. 1955. Multiple Range and Multiple F Tests. Biometrics, 11 :1- 41.
- Levtchenko.E. 2014. performs consultancy for Orphan Europe, Chiesi, Kyowa Kirin, Advicenneand was supported by a research grant from Horizon Pharma.
- Liu, Z.J., Cordes, J., 2016. DNA marker technologies and their applications in aquaculture genetics. Aquaculture 238, 1–37.
- Mohammadabadi M.; F. Bordbar; J. Jensen ; M. Du and W. Guo. 2021. Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals. Farm Animals. Animals 2021, 11, 835. https://doi.org/10.3390/ani11030835.
- NCBI Resource Coordinators (2023). "Database resources of the National Center for Biotechnology Information". Nucleic Acids Research 41 (Database issue): D8–D20.
- Ropka-Molik, K.; Pawlina-Tyszko, K.; Z˙ ukowski, K.; Piórkowska, K.; Z˙ ak, G.; Gurgul, A.; Derebecka, N.; Wesoły, J.2018. Examining the Genetic Background of Porcine Muscle Growth and Development Based on Transcriptome and miRNAome Data. Int. J. Mol. Sci. 2018, 19, 1208. [CrossRef].
- Sambrook, J. and D. Russell.2001. Molecular Cloning: A Laboratory Manual. 3rd ed. Cold Spring Harbor. New York. USA.
- SAS. 2016. Statistical Analysis System, User's Guide. Statistical. Version 9. 1th ed. SAS. Inst. Inc. Cary. N.C. USA.
- Yi, X.; Tao, Y.; Lin, X.; Dai, Y.; Yang, T.; Yue, X.; Jiang, X.; Li, X.; Jiang, D.; Andrade, K.C.; et al. 2017. Histone methyltransferase Setd2 is critical for the proliferation and differentiation of myoblasts. Biochim. Biophys. Acta Mol. Cell Res., 1864, 697–707. [CrossRef].
- Zhang D, Bai J, Ma Z, Ma X, Cao X, Li F.2016. Regulatory roles of adiponectin receptor 1 and 2 in sheep preadipocytes during adipocyte differentiation. Ital J Anim Sci. 2019;18:704–12.
his study investigated the association between single-nucleotide polymorphisms (SNPs) in the cysteine- and
glycine-rich protein 2 gene (CSRP2) and body dimensions in Awassi sheep. Blood samples and measurements of body length,
front height, and back height were collected from 50 animals raised at a private station in Babylon Province, Iraq, during
2022–2023. Genomic DNA was extracted, and exon 7, together with the adjacent terminal region, was amplified and
sequenced. Allele and genotype frequencies were assessed using the chi-square test, while genotype effects were analyzed
with a general linear model followed by Duncan’s multiple range test. Two C>T substitutions were identified at positions
20315 and 20094, producing the CC, CT, and TT genotypes. The T allele was the most frequent at both positions, with
frequencies of 0.67 at position 20315 and 0.57 at position 20094.
Keywords :
Awassi Sheep; CSRP2 Gene; Single Nucleotide Polymorphism; Body Dimensions; Growth Traits; Marker-Assisted Selection.