Authors :
Marsintha L.MTampubolon; Cut Nurliza; Trimurni Abidin; Saharman Gea; Basri A. Gani
Volume/Issue :
Volume 7 - 2022, Issue 7 - July
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3vBbujk
DOI :
https://doi.org/10.5281/zenodo.6958063
Abstract :
Streptococcus mutans (S. mutans) was
reported as the primary agent of dental caries. The
Glucosyltransferase (GTF) enzyme produced by S.
mutans contributes to the virulence factor by converting
sucrose into fructose and glucans used in biofilm
formation. Probiotics such as yogurt and kefir of
bacteria could also affect the oral ecology and
potentially inhibit the GTF enzyme produced by
S.mutans. The assessment of probiotic yogurt and kefir
to inhibition of glucosyltransferase enzyme of
Streptococcus mutansATCC25175.This research was
done in an experimental post-test-only group design
using Microtiter Plate Assay by measuring the Optical
Density (OD) of the ELISA reader. Two aspects were
analyzed; first, the evaluation of probiotic products
concerning their biomass index, biostability, and pH
alteration; second, the assessment of GTF enzyme
activity and fructose release by S.mutans.
Keywords :
Dental Caries, Glucosyltransferase, Probiotic, Streptococcus mutans.
Streptococcus mutans (S. mutans) was
reported as the primary agent of dental caries. The
Glucosyltransferase (GTF) enzyme produced by S.
mutans contributes to the virulence factor by converting
sucrose into fructose and glucans used in biofilm
formation. Probiotics such as yogurt and kefir of
bacteria could also affect the oral ecology and
potentially inhibit the GTF enzyme produced by
S.mutans. The assessment of probiotic yogurt and kefir
to inhibition of glucosyltransferase enzyme of
Streptococcus mutansATCC25175.This research was
done in an experimental post-test-only group design
using Microtiter Plate Assay by measuring the Optical
Density (OD) of the ELISA reader. Two aspects were
analyzed; first, the evaluation of probiotic products
concerning their biomass index, biostability, and pH
alteration; second, the assessment of GTF enzyme
activity and fructose release by S.mutans.
Keywords :
Dental Caries, Glucosyltransferase, Probiotic, Streptococcus mutans.
