Streptococcus mutans (S. mutans) was reported as the primary agent of dental caries. The Glucosyltransferase (GTF) enzyme produced by S. mutans contributes to the virulence factor by converting sucrose into fructose and glucans used in biofilm formation. Probiotics such as yogurt and kefir of bacteria could also affect the oral ecology and potentially inhibit the GTF enzyme produced by S.mutans. The assessment of probiotic yogurt and kefir to inhibition of glucosyltransferase enzyme of Streptococcus mutansATCC25175.This research was done in an experimental post-test-only group design using Microtiter Plate Assay by measuring the Optical Density (OD) of the ELISA reader. Two aspects were analyzed; first, the evaluation of probiotic products concerning their biomass index, biostability, and pH alteration; second, the assessment of GTF enzyme activity and fructose release by S.mutans.

Keywords : Dental Caries, Glucosyltransferase, Probiotic, Streptococcus mutans.