Fixation is an initial and essential step in diagnostic cascade for cytological specimen, in usual it carried out by using 95% Ethyl Alcohol, but this alcohol based fixation method exhibited some limitations and problems either for specimen, workers, and even the environment. New way to find ecofriendly and specimen safe alternative to alcohol is critical, the suggestion says Bees Honey is most suitable alternative. The study objective was to investigate the efficiency of using bees honey instead of ethanol fixation in buccal smear samples. Samples were collected by gently scraping donor’s buccal mucosa by wooden sticks (n=60) and divided into alcohol group coded (E) and honey group coded (H). Honey group divided according to concentration into: A (10%), B (20%) and C (30%), then both groups fixed for 15 min after that stained with papanicolaou staining procedure, then examined under light microscope. The results show the honey fixed smears were same as the result of ethanol fixed ones. And the analysis of staining result which done by using Chi square analysis program the results showed good observation of cytoplasmic staining result obtained by using 10% honey fixative agent. So honey could be used as excellent alternative to ethanol cytofixative.

Keywords : Alternative Fixative, Ethyl Alcohol (Ethanol) , Cytology, Honey.