Authors :
Esraa Hassan; Tassnim Abdalrhman; Aisha Osman; Musa Omer; Lubna Elnajeb; Maissa Kamal
Volume/Issue :
Volume 8 - 2023, Issue 2 - February
Scribd :
https://bit.ly/3yEtwSJ
DOI :
https://doi.org/10.5281/zenodo.7735441
Abstract :
Fixation is an initial and essential step in
diagnostic cascade for cytological specimen, in usual it
carried out by using 95% Ethyl Alcohol, but this alcohol
based fixation method exhibited some limitations and
problems either for specimen, workers, and even the
environment. New way to find ecofriendly and specimen
safe alternative to alcohol is critical, the suggestion says
Bees Honey is most suitable alternative. The study
objective was to investigate the efficiency of using bees
honey instead of ethanol fixation in buccal smear
samples. Samples were collected by gently scraping
donor’s buccal mucosa by wooden sticks (n=60) and
divided into alcohol group coded (E) and honey group
coded (H). Honey group divided according to
concentration into: A (10%), B (20%) and C (30%), then
both groups fixed for 15 min after that stained with
papanicolaou staining procedure, then examined under
light microscope. The results show the honey fixed
smears were same as the result of ethanol fixed ones.
And the analysis of staining result which done by using
Chi square analysis program the results showed good
observation of cytoplasmic staining result obtained by
using 10% honey fixative agent. So honey could be used
as excellent alternative to ethanol cytofixative.
Keywords :
Alternative Fixative, Ethyl Alcohol (Ethanol) , Cytology, Honey.
Fixation is an initial and essential step in
diagnostic cascade for cytological specimen, in usual it
carried out by using 95% Ethyl Alcohol, but this alcohol
based fixation method exhibited some limitations and
problems either for specimen, workers, and even the
environment. New way to find ecofriendly and specimen
safe alternative to alcohol is critical, the suggestion says
Bees Honey is most suitable alternative. The study
objective was to investigate the efficiency of using bees
honey instead of ethanol fixation in buccal smear
samples. Samples were collected by gently scraping
donor’s buccal mucosa by wooden sticks (n=60) and
divided into alcohol group coded (E) and honey group
coded (H). Honey group divided according to
concentration into: A (10%), B (20%) and C (30%), then
both groups fixed for 15 min after that stained with
papanicolaou staining procedure, then examined under
light microscope. The results show the honey fixed
smears were same as the result of ethanol fixed ones.
And the analysis of staining result which done by using
Chi square analysis program the results showed good
observation of cytoplasmic staining result obtained by
using 10% honey fixative agent. So honey could be used
as excellent alternative to ethanol cytofixative.
Keywords :
Alternative Fixative, Ethyl Alcohol (Ethanol) , Cytology, Honey.
