Antibiotic resistance is adversely affecting
public health. The excess and overuse of antibiotics leads
to the growth of antibiotic resistant Staphylococcus
aureus (MRSA) in the hospital environment. Raw tank
of sewage treatment plant receives pathogen from all
over the units of hospital. In this study MRSA was
isolated from raw tank and identified by using mannitol
salt agar and Kirby Bauer disc diffusion test. Yellow
colonies on mannitol salt agar with resistance zone on
cefoxitin, oxacillin and vancomycin confirmed the
resistance of MRSA according to the guidelines of
clinical laboratory standards institute.
Keywords :
Antibiotic Resistance, Staphylococcus aureus, Mannitol Salt Agar, Kirby Bauer Disc Diffusion Test.