The Evaluation of the Antidiarrheal Properties of Ethanolic Rhizome Extract of Zingiber zerumbet (Zingiberaceae)


Authors : Raudha D. Abdulrauf; Junnin Gay L. Garay; Johayber M. Macud; Sophia C. Magbanua; Princess Nhurliza S. Ramber

Volume/Issue : Volume 10 - 2025, Issue 6 - June

Google Scholar : https://tinyurl.com/yc4amwek

DOI : https://doi.org/10.38124/ijisrt/25jun1866

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Abstract : Diarrhea was a prevalent condition that affected millions globally each year, representing a significant public health challenge. It often led to acute dehydration, with severe complications particularly impacting vulnerable populations such as the elderly and young children. The condition remained a leading cause of morbidity and mortality, especially in resource- limited settings. Zingiber zerumbet, commonly referred to as shampoo ginger, was a member of the Zingiberaceae family, closely related to Z. officinale (ginger). Traditionally used in medicine, Z. zerumbet was believed to possess antidiarrheal potential due to its rich phytochemical profile, which included anti-inflammatory and antimicrobial compounds. Research on Z. officinale has demonstrated its efficacy in managing diarrhea through mechanisms such as reducing intestinal motility and addressing microbial pathogens. It was hypothesized that Z. zerumbet may exert similar effects, potentially mitigating excessive intestinal secretions and resolving microbial factors contributing to diarrhea. This study investigated the antidiarrheal activity of ethanolic rhizome extract of Zingiber zerumbet using castor oil- induced diarrhea and gastrointestinal motility tests in mice. The extract was administered at 25%, 50%, and 75% of the LD50, with loperamide as the positive control and PNSS with 2% Tween 80 as the negative control. Evaluated parameters included stool frequency, consistency, and intestinal transit using the charcoal meal method. The extract was consistently prepared through ethanol maceration and demonstrated a favorable safety profile, with no signs of toxicity at the administered doses. Pharmacological assessments revealed significant reduction of diarrhea frequency, improvement of stool consistency, and inhibition of gastrointestinal motility compared to controls. These outcomes led to the rejection of the null hypotheses and supported the alternative, confirming a dose-dependent antidiarrheal effect comparable to loperamide. The findings underscore the potential of Z. zerumbet as a safe and effective natural antidiarrheal agent, warranting further exploration for therapeutic use.

Keywords : Zingiber zerumbet, Antidiarrheal, Ethanolic Extract, Rhizome, Castor Oil-Induced Test, Gastrointestinal Motility Test.

References :

  1. Acharya R, Prakash RO, Kumar MS. 2015 Apr 28. Zingiber Zerumbet (L.) sm., a reservoir plant for therapeutic uses: A Review. Academia.edu. [accessed 2024 Oct 6]. https://www.academia.edu/12132907/Zingiber_zerumbet_ L_Sm_a_reservoir_plant_f or_therapeutic_uses_A_Review
  2. Adriana Y. Koga, Flávio L. Beltrame, Airton V. Pereira. 2016. Several aspects of Zingiber    zerumbet, a review. Available from: http://www.ScienceDirect.com/science/article/pii/S0102695X1600025
  3. Afrin SR, Islam MR, Khanam BH, Proma NM, Didari SS, Jannat SW, Hossain MK. 2021. Phytochemical and pharmacological investigations of different extracts of leaves and stem barks of Macropanax dispermus (Araliaceae): A promising ethnomedicinal plant. Future Journal of Pharmaceutical Sciences. 7(1). doi:10.1186/s43094-021- 00313-4
  4. Akhondi H, Simonsen KA. 2023 Aug 7. Bacterial diarrhea. StatPearls [Internet]. [accessed 2024 Oct 6]. https://www.ncbi.nlm.nih.gov/books/NBK551643/
  5. Alolga RN, Wang F, Zhang X, Li J, Tran L-SP, Yin X. 2022 Jun 28. Bioactive compounds from the Zingiberaceae family with known antioxidant activities for possible therapeutic uses. MDPI. [accessed 2024 Oct 5]. https://www.mdpi.com/2076- 3921/11/7/1281
  6. Anjankar MP. 2023 Sep. (PDF) OECD Guidelines for Acute Oral Toxicity Studies: An overview. research gate . [accessed 2024 Oct 7]. https://www.researchgate.net/publication/373895537_OECD_GUIDELINES_FOR_A CUTE_ORAL_TOXICITY_STUDIES_AN_OVERVIEW
  7. Arasaradnam RP, Brown S, Forbes A, Fox MR, Hungin P, Kelman L, Major G, O’Connor M, Sanders DS, Sinha R, et al. 2018 August. Guidelines for the investigation of chronic diarrhea in adults: British Society of Gastroenterology, 3rd Edition. Gut. [accessed 2024 Oct 6]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204957/
  8. Assiry AA, Ahmed N, Abdulmajeed Almuaddi, Saif A, Mohammed Abdulrahman Alshahrani, Roshan Noor Mohamed, Mohmed Isaqali Karobari. 2023. The antioxidant activity, preliminary phytochemical screening of Zingiber zerumbet and antimicrobial efficacy against selective endodontic bacteria. Food Science and Nutrition. 11(8):4853– 4860. doi: https://doi.org/10.1002/fsn3.3462.
  9. Azam G, Noman S, Pavel AM. Evaluation of anti-diarrhoeal activity of Curcuma zedoaria rhizome. Journal of Pharmacognosy and Phytochemistry. 2017 Apr 6 [accessed                                                       2024                                             Nov  18]. https://www.phytojournal.com/archives?year=2017&vol=6&issue=3&ArticleId=1206 &si=false
  10. Bahishti AA. 2022 Mar 1. The vital role of research methodology in addressing the research questions. International Journal of Methodology. [accessed 2024 Oct 23]. https://journals.aijr.org/index.php/ijm/article/view/114#pkp_content_footer 4
  11. Baig I, Veerashekar T, Shekshavali T, Anjum E, Nagaraja HR, Ruchitha V. Antidiarrheal Activity of Leucaena leucocephala in Castor Oil Induced Diarrhea in Albino Rats. Res Rev J Drug Des Discov. 2020;7(1):8–12. Available from: https://pharmajournals.stmjournals.in/index.php/RRJoDDD/article/view/716
  12. Bardal S, Waechter J, Martin D. 2011. Applied Pharmacology e-book. Google Books. [accessed        2024       Oct          6]. https://books.google.com.ph/books?hl=en&lr=&id=CZZ6YpzVA0UC&oi=fnd&pg=P P1&ots=4X251yjJhM&sig=wjHx3 AXwLJYzVrQbatz2O9YqBpM&redir_esc=y#v=o nepage&q&f=false
  13. Chan HT, Siu Kuin Wong, Eric Wei. 2017. Alpinia zerumbet, a ginger plant with a multitude of medicinal properties: An update on its research findings. 26(11). doi: https://doi.org/10.5246/jcps.2017.11.088.
  14. Chan JSW, Lim XY, Japri N, Ahmad IF, Tan TYC. 2024. Zingiber zerumbet: A Scoping Review of its Medicinal Properties. Planta Med. 2024 Mar;90(3):204-218. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10869203/
  15. Chan Y, Lee J, Wong S, Tan Y, Lim W. 2023. Zerumbone from Zingiber zerumbet: a comprehensive review of its pharmacological activities. Molecules. 28(1):409. Available from: https://www.mdpi.com/1420-3049/28/1/409
  16. Chavan J, Misra R. 2023. Zingiber zerumbet (L.) Roscoe ex Sm.: biotechnological advancements and perspectives. Available from:  https://www.researchgate.net/publication/372527075_Zingiber_zerumbet_L_ Roscoe_ex_Sm_biotechnological_ advancements_and_perspectives
  17. Dash P, Nasrin M, Raihan S, Ali M. 2014. Study of antidiarrhoeal activity of two medicinal plants of Bangladesh in castor-oil induced diarrhea. Int J Pharm Sci Res. 2014;5(9):3864-3868. doi: 10.13040/IJPSR.0975-8232.5(9).3864-68
  18. Daswani PG, Brijesh S, Tatali P, Antia NH, Birdi TJ. 2010. Antidiarrhoeal activity of Zingiber officinale (Rosc.). Curr Sci. 2010;98(2):222-229. Available from: https://www.jstor.org/stable/24111513
  19. Degu A, Kefale B, Alemayehu D, Tegegne GT. 2020. Evaluation of the Antidiarrheal Activity of Hydromethanol Crude Extracts of Ruta chalepensis and Vernonia amygdalina in Mice. Evidence-Based Complementary and Alternative Medicine. 2020:1–6. doi:https://doi.org/10.1155/2020/8318713.
  20. Desta GT, Adela Alemu M, Tsegaw A, Belete TM, Adugna BY. 2021. Antidiarrheal Effect of 80% Methanol Extract and Fractions of Clerodendrum myricoides (Hochst.) Vatke (Lamiaceae) Leaf in Swiss Albino Mice. Uzor PF, editor. Evidence-Based Complementary and              Alternative Medicine. 2021:1–10. doi: https://doi.org/10.1155/2021/9369173.
  21. Doe P, Mshelia VC, Otchere DB, Fofana O, Amissah AA, Yeboah PN, Mills HNL, Ansah-Abrokwah E, Ametepey NK. 2019 Nov 6. Anti-diarrheal activity of ethanol and chloroform seed extract of cola nitida in experimentally induced diarrhea. Universal Journal of Pharmaceutical Research. doi:https://doi.org/10.22270/ujpr.v4i5.312. [accessed 2024 Nov 11]. https://pdfs.semanticscholar.org/0019/7949616b8c010e70ea278f603184a9e92c36.pdf?_ga=2.156007547.1138082993.1625843806-1028080036.1610065298.
  22. Evangelista S. Charcoal Meal, an Old Test But Still Valid to Measure Intestinal Transit in vivo: Effect of Activation of Tachykinin NK-2 Receptors. 2011. [accessed 2024 Oct 6]. https://www.researchgate.net/publication/276274559 _Charcoal_Meal_an_Old_Test_But_Still_Valid_to_Measure_Intestinal_Transit_in_vivo_Effect_of_Activation_of_Ta chykinin_NK-2_Receptors#full-text
  23. Fadilah IN, Kurniawan H. 2022 March. Evaluation of Use Of Diarrhea Drug in Pediatric Patients at Dolat Rayat Health Center Karo District. Medalion Journal. [accessed 2024 Oct]. https://medalionjournal.com/index.php/go/article/view/22
  24. Fokam Tagne MA, Akaou H, Noubissi PA, Foyet Fondjo A, Rékabi Y, Wambe H, Kamgang R, Essame Oyono J-L. Effect of the Hydroethanolic Extract of Bixa orellana Linn (Bixaceae) Leaves on Castor Oil-Induced Diarrhea in Swiss Albino Mice. Gastroenterology Research and Practice. 2019;2019:1–8. doi:10.1155/2019/6963548
  25. Ghazalee NS, Jantan I, Arshad L, Haque MA. 2019. Immunosuppressive effects of the standardized extract of Zingiber zerumbet on innate immune responses in Wistar rats. Phytother Res. 2019;33(4):929-938. https://doi.org/10.1002/ptr.6285
  26. Giacosa A, Morazzoni P, Bombardelli E, Riva A, Bianchi Porro G, Rondanelli M. 2015. Can nausea and vomiting be treated with ginger extract? Eur Rev Med Pharmacol Sci. 19(7):1291-1296. Available from: https://www.europeanreview.org/ wp/wp- content/uploads/1291- 1296.pdf
  27. Gotfried J. Diarrhea - diarrhea. 2024 May. Merck Manual Professional Edition. [accessed                          2024                Nov        11]. https://www.merckmanuals.com/professional/gastrointestinal-disorders/symptoms-of- gastrointestinal-disorders/diarrhea
  28. Gudeta BM, Taye GM, Abula T, Gadisa DA. 2020. Evaluation of Anti-Diarrheal Activity of 80% Methanol Extracts of Vernonia amygdalina Delile (Asteraceae) Leaves in          Mice.      Journal   of            Experimental        Pharmacology. doi: https://doi.org/10.2147//JEP.S282669
  29. Guide for the care and use of Laboratory Animals. 2011 [accessed 2024 Oct 23]. https://grants.nih.gov/grants/olaw/guide-for-the-care-and-use-of-laboratory- animals.pdf
  30. Haniadka R, Saldanha E, Sunita V, Palatty PL, Fayad R, Baliga MS. 2013. A review of the gastroprotective effects of ginger (Zingiber officinale Roscoe). Food Funct. 4(6):845-855. Available from: https://pubs.rsc.org/en/content/articlelanding/2013/ fo/c3fo30337c/unauth
  31. Iqbal E, Salim KA, Lim LBL. 2015. Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. Journal of King Saud University - Science. 27(3):224–232. doi:https://doi.org/10.1016/j.jksus.2015.02.003.
  32. Kamadyaapa DR, Gondwe MM, Shauli M, Rusike CS, Iputo J. 2018. Evaluation of antidiarrheal activity of ethanolic leaf extract of Eriobotrya Japonica Lindl. Asian Journal of Pharmaceutical and Clinical Research. 11(9):502. doi:10.22159/ajpcr.2018.v11i9.27256
  33. Kharal S, Siddique F, Arshad M, Iftikhar K, Muhammad Tahir Akhtar, Qadir R, Sameeh MY, Muhammad Mustaqeem, Zhuang J, Xia L. 2023. Assessment of phytochemicals, antioxidant properties, and in vivo antidiarrheal activity of date palm (Phoenix dactylifera L.). International Journal of Food Properties. 26(1):2243–2255. doi:https://doi.org/10.1080/10942912.2023.2244684.
  34. Kifle ZD, Atnafie SA, Yimer Tadesse T, Belachew TF, Kidanu BB. 2021. Methanolic Crude Extract of Hagenia abyssinica Possesses Significant Antidiarrheal Effect: Evidence for In Vivo Antidiarrheal Activity. Mancianti F, editor. Evidence-Based
  35. Complementary and Alternative Medicine. 2021:1–8. doi:https://doi.org/10.1155/2021/9944629.
  36. Koga AY, Beltrame FL, Pereira AV. Several aspects of Zingiber zerumbet: a review [Internet]. Available from: https://www.sciencedirect.com/science/article/pii/S0102695X16000259?via%3Dihub Lempuyang (Zingiber zerumbet, Zingiber aromaticum, Zingiber americans). Open Access Maced J Med Sci. 2022;10(A):695–701. https://doi.org/10.3889/ oamjms.2022.9132
  37. Li Y, Xia S, Jiang X, Feng C, Gong S, Ma J, Fang Z, Yin J, Yin Y. 2021. Gut microbiota and diarrhea: an updated review. Front Cell Infect Microbiol. 11:625210. Available from: https://doi.org/10.3389/fcimb.2021.625210
  38. Libretexts. 2023 Feb 3. 7.4: Antidiarrheal medications and laxatives. Medicine LibreTexts. [accessed 2024 Oct 6]. https://med.libretexts.org/Bookshelves/Nursing/Nursing_Pharmacology_(OpenRN)/0 7%3A_Gastrointestinal/7.04%3A _Antidiarrheal_Medications_and_Laxatives#title
  39. Liu Q, Liu J, Liu M. 2024 Aug 15. Association of drinking water services with the disease burden of diarrhea in children under five in 200 countries from 2000 to 2021. Science        Direct.    [accessed 2024 Oct]. https://www.sciencedirect.com/ science/article/pii/S2949790624002763
  40. Manjunath LN, Shivkumar H, Rimaben P, Bhargava B. 2011. (PDF) antidiarrhoeal activity of leaf extract of moringa oleifera in experimentally induced diarrhoea in rats. [accessed 2024 Oct      6]. https://www.researchgate.net/publication/221658755_ Antidiarrhoeal_Activity_of_Le af_Extract_of_Moringa_Oleifera_in_Experimentally_Induced_Diarrhoea_in_Rats
  41. Margolis KG, Picoraro JA. Development of gastrointestinal motility. Fetal and Neonatal Physiology (Fifth Edition). 2016 Jul 6 [accessed 2024 Nov 17]. https://www.sciencedirect.com/science/article/abs/pii/B9780323352147000901
  42. Meite S, N’guessan J, Bahi C, Yapi H, Djaman A, Guina F. 2009. Antidiarrheal activity of the ethyl acetate extract of Morinda Morindoides in rats. Tropical Journal of Pharmaceutical               Research.              [accessed               2024       Oct 6]. https://www.ajol.info/index.php/tjpr/article/view/44533#:~:text=The%20extract%20 %281000%20mg%2Fkg%29%20 produced%20a%20decrease%20in,of%20up%20to%20a%20dose%20of%206000%20mg%2Fkg.
  43. Mengesha AK, Birru EM, Adugna M. 2022 Nov 15. Anti-diarrheal activities of hydromethanolic crude extract and solvent fractions of acacia seyal (fabaceae) roots in mice. Clinical pharmacology : advances and applications. [accessed 2024 Oct 23]. https://pmc.ncbi.nlm.nih.gov/articles/PMC9675325/#s0002
  44. Mirza K. Anti-diarrheal effects of methanol extract of Curcuma longa. 2019. [Internet]. Available from: https://www.researchgate.net/publication/335527429_Original_article_Anti- diarrheal_effects_of_Methanol_extract_of_ Curcuma_longa
  45. Murugan S, Purusothaman D, Richard EJ, Chalichem NSS, Bethapudi B, Chandrasekaran PR, Velusami CC, D’Souza P, Mundkinajeddu D. 2020. Anti- diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms. Avicenna journal of phytomedicine. [accessed 2024 Oct 6]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7430962/#B16
  46. Mussarat S, Adnan M, Begum S, Alamgeer, Ullah R, Kowalczyk A. 2022. In vivo efficacy, toxicity assessment, and elemental analysis of traditionally used polyherbal recipe for diarrhea. Evid Based Complement Alternat Med. 2022:5977795. Available from: https://doi.org/10.1155/2022/5977795
  47. Nemeth V., Pfleghaar N. 2022 Nov 21. Diarrhea. StatPearls [Internet]. [accessed 2024 Oct 6]. https://www.ncbi.nlm.nih.gov/books/NBK448082/
  48. Norulaini NAN, Anuar OA, Omar AKM, AlKarkhi AFM, Setianto WB, Fatehah MO, Sahena F, Zaidul ISM. 2008 Sep 30. Optimization of SC–CO2 extraction of Zerumbone from Zingiber Zerumbet (L) smith. Food Chemistry. [accessed 2024 Oct 6]. https://www.sciencedirect.com/science/article/abs/pii/S0308814608011667
  49. Okamoto S, Yu F, Harada H, Okajima T, Hattan J, Misawa N, Utsumi R. 2011. A short- chain dehydrogenase involved in terpene metabolism ... The Febs Journal. [accessed 2024 Oct 6]. https://www.researchgate.net/publication/ 51214871_A_short- chain_dehydrogenase_involved_in_terpene_metabolism_from_Zingiber_zerumbet Patil D, Kotwal S. 2020 Apr 16. Medicinal Plants Profile and Some Screening Method Used in the Treatment of Diarrhea: A Review. Google scholar. [accessed 2024 Oct 5]. https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&as_ylo=2017&as_yhi=20 24&q=MEDICINAL+PLANTS+PROFILE+AND+SOME+SCREENING+METHOD+USED+IN+THE+TREATMENT+OF+DIARRHEA%3A+A+REVIEW+D.+S.+Patil+and+S.+V.+Kotwal&btnG=https://wjpr.s3.ap-south-1.amazonaws.com/ article_issue/1588154772.face
  50. Petrovska BB. 2012 Jan. Historical Review of Medicinal Plants’ usage. Pharmacognosy reviews. [accessed 2024 Oct 6]. https://www.ncbi.nlm.nih.gov/pmc/ articles/PMC3358962/
  51. Philippine Statistics Authority. 2021. Annual incidence of diarrheal diseases in the Philippines, 2010-2019. Available from: https://psa.gov.ph 12
  52. Pinheiro C, Pinheiro D, Seixas M, Danniel C, Amadis F, de Elisson T, Tayah I, Panizza 2019. Phytochemical analysis and antinociceptive activity of bitter ginger (Zingiber zerumbet) cultivated in Manaus/Amazonas. Afr J Biotechnol. 2019;18(5):257-264. doi: 10.5897/AJB2018.16722
  53. Preshahdin NA, Jani NA, Iberahim R. 2023 Feb 28. Phytochemicals and antibacterial activity of zingiber zerumbet growing in Negeri Sembilan, Malaysia. Journal of Science and      Mathematics        Letters.   [accessed               2024       Oct          6]. https://ejournal.upsi.edu.my/index.php/JSML/article/view/7140
  54. Preshahdin NA, Jani NA, Iberahim R. 2023. Phytochemicals and antibacterial activity of Zingiber zerumbet growing in Negeri Sembilan, Malaysia. J Sci Math Lett. 11(1):20–29. Available from: https://doi.org/10.37134/jsml.vol11.1.3.2023.
  55. Rahman MdK, Chowdhury MdA, Islam MT, Chowdhury MdA, Uddin ME, Sumi CD. Evaluation of Antidiarrheal Activity of Methanolic Extract of Maranta arundinacea Linn. Leaves. Advances in Pharmacological Sciences. 2015;2015:1–6. doi:10.1155/2015/257057
  56. Ramadanil, Damry, Rusdi, Hamzah B, Zubair MS. 2019. Traditional usages and phytochemical screenings of selected Zingiberaceae from Central Sulawesi, Indonesia. Pharmacogn J. 11(3):505–510. Available from: https://doi.org/10.5530/pj.2019.11.80.
  57. Ramzan A, Mahmood T, Qureshi N, Latif R. 2023. Antidiarrheal activity of Zingiber zerumbet rhizome extract and its essential oils in murine models. J Med Plant Res. Available from: https://pubmed.ncbi.nlm.nih.gov/38035621/
  58. Ramzan M, Zeshan B. 2023. Assessment of the Phytochemical Analysis and Antimicrobial Potentials of Zingiber zerumbet. Molecules. 2023;28(1):409. https://doi.org/10.3390/molecules28010409
  59. Ramzan M, Zeshan B. 2023. Assessment of the phytochemical analysis and antimicrobial potentials of Zingiber zerumbet. Molecules. 28(409). Available from: https://doi.org/10.3390/molecules28010409.
  60. Rosseels M.L, Fleurance R, Goody SMG. 2018 Sep 18. The Impact of Drug-Induced Effects on the Gastrointestinal System: Challenges and Issue Resolution for Safety Pharmacology. Science Direct. [accessed 2024 Oct]. https://www.sciencedirect.com/science/article/abs/pii/B9780128122068000054
  61. Saravanan C, Biswas B. 2018 May 23. Introductory chapter: An outline of chemical reagents and reactions in inorganic synthesis. IntechOpen. [accessed 2024 Oct 23]. https://www.intechopen.com/chapters/60218
  62. Schiller LR. 2017. Antidiarrheal drug therapy. Current gastroenterology reports. [accessed 2024 Oct 6]. https://pubmed.ncbi.nlm.nih.gov/28397130/
  63. Shahrul. 2022 Nov 7. Lempoyang Rhizome Zingiber zerumbet (L.) Smith. Global Information Hub On Integrated Medicine. [accessed 2024 Nov 11]. https://globinmed.com/medicinal_herbs/zingiber-zerumbet-l-smith2/.
  64. Shaikh JR, Patil M. 2020. Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies. 8(2):603–608. doi:https://doi.org/10.22271/chemi.2020.v8.i2i.8834.
  65. Sharma T, Pandey B, Shrestha BK, Koju GM, Thusa R, Karki N. 2020. Phytochemical screening of medicinal plants and study of the effect of phytoconstituents in seed germination. Tribhuvan Univ J. 35(2):1–11. Available from: https://doi.org/10.3126/tuj.v35i2.36183.
  66. Shetu HJ, Taskina KT, Sikta SA, Anwar R, Bin Rashed SS, Dash PR. (PDF) pharmacological importance of Kaempferia Galanga (Zingiberaceae): A Mini Review. 2018 May [accessed 2024 Nov                18]. https://www.researchgate.net/publication /330117569_Pharmacological_importance_o f_Kaempferia_galanga_Zingiberaceae_A_mini_review
  67. Sidahmed HMA, Hashim NM, Abdulla MA, Ali HM, Mohan S, Abdelwahab SI, Taha MME, Fai LM, Vadivelu J. 2015. Antisecretory, gastroprotective, antioxidant and anti- helicobcter pylori activity of zerumbone from Zingiber Zerumbet (L.) smith. PLOS ONE.              [accessed 2024 Oct 6]. https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0121060
  68. Soumya T, Jayasree PR, Kumar PRM. 2023 Aug 31. Zingiberaceae plants: A cornucopia of promising chemotherapeuticals for cancer cure. SpringerLink. [accessed 2024 Oct 5]. https://link.springer.com/chapter/10.1007/978-3-031-28780-0_16
  69. Stone C. 2020 May 30. AGA releases guideline on the evaluation of chronic diarrhea. American        Gastroenterological Association. [accessed 2024 Nov 11]. https://gastro.org/press-releases/aga-releases-guideline-on-the-evaluation-of-chronic- diarrhea-1
  70. Su D-M, Long J-B, Liu X-P, Li S-B. 2019 Mar 18. Anti-diarrhea effects of polysaccharides from Zingiber officinale rhizome on a rat diarrhea model. Tropical Journal of Pharmaceutical Research. [accessed 2024 Oct 5]. https://www.ajol.info/index.php/tjpr/article/view/184758
  71. Susanti I, Rimadani Pratiwi, Yudi Rosandi, Aliya Nur Hasanah. 2024. Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate. Plants. 13(7):965–965. doi:https://doi.org/10.3390/plants13070965.
  72. Sweetser S. 2012 Jun. Evaluating the patient with diarrhea: A case-based approach. Mayo Clinic      proceedings. [accessed 2024 Oct 6]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3538472/.
  73. Tadesse WT, Hailu AE, Gurmu AE, Mechesso AF. 2014. Experimental assessment of antidiarrheal and antisecretory activity of 80% methanolic leaf extract of Zehneria scabra in mice. BMC Complementary and Alternative Medicine. 14(1). doi: https://doi.org/10.1186/1472-6882-14-460.
  74. Tafesse G, Mekonnen Y. Anti-diarrheal activity of leaf extract of Juniperus procera and its effect on intestinal motility in albino mice. Journal of Medical and Biomedical Sciences. 2012 [accessed 2024 Oct 23]. https://www.ajol.info/index.php/ jmbs/article/view/82742#:~:text=The%20aqueous%2 0extract%20of%20J.%20procera%20significantly%20%28p,cm%20 when%20treated %20with%20300%20mg%20kg%20-1.
  75. Teklie Mengie Ayele, Endeshaw Chekol Abebe, Zelalem Tilahun Muche, Melaku Mekonnen Agidew, Yohannes Shumet Yimer, Getu Tesfaw Addis, Nega Dagnaw Baye, Achenef Bogale Kassie, Muluken Adela Alemu, Tesfagegn Gobezie Yiblet, et al. 2023. In vivo antidiarrheal activity of the crude extract and solvent fractions of Rhamnus prinoides (Rhamnaceae) leaves. Heliyon. 9(6):e16654–e16654. doi:https://doi.org/10.1016/j.heliyon.2023.e16654.
  76. Tessema Desta G, Andargie Ferede Y, Sisay Zewdu W, Adela Alemu M. 2022. Evaluation of Antidiarrheal Activity of 80% Methanol Extract and Solvent Fractions of the Leaves of Withania somnifera (L.) Dunal in Swiss Albino Mice. Andrade-Cetto A, editor. Evidence-Based Complementary and Alternative Medicine. 2022:1–11. doi:https://doi.org/10.1155/2022/7968973.
  77. Tewtrakul S, Subhadhirasakul S. 2006 Aug 15. Anti-allergic activity of some selected plants in the Zingiberaceae family. Journal of Ethnopharmacology. [accessed 2024 Oct 6]. https://www.sciencedirect.com/science/article/abs/pii/ S0378874106004077
  78. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. 2022 Nov 7. Phytochemical screening and Extraction: A Review. GlobinMed. [accessed 2024 Nov 11]. https://globinmed.com/medicinal_herbs/zingiber-zerumbet-l-smith2/.
  79. United States Department of Agriculture. Zingiber zerumbet classification [Internet]. Available from: https://plants.usda.gov/home/classification/31157
  80. Wang S, Zhao Y, Zhang J, Huang X, Wang Y, Xu X, Zheng B, Zhou X, Tian H, Liu L, Mei Q. 2015. Antidiarrheal effect of Alpinia oxyphylla Miq. (Zingiberaceae) in experimental mice and its possible mechanism of action. J Ethnopharmacol. 2015;168:182-190. https://doi.org/10.1016/j.jep.2015.03.066. Available from: https://www.sciencedirect.com/science/ article/pii/S037887411500225Widyastiwi W, Roseno M. 2022. Anxiolytic Activity of Ethanolic Extract of Three Species of Indonesian Lempuyang (Zingiber zerumbet, Zingiber aromaticum, and Zingiber americans) https://oamjms.eu/index.php/mjms/article/view/9132
  81. World Health Organization. 2017 May 2. Diarrhoeal disease. Available from: https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease
  82. World Health Organization. 2024. Diarrhea Disease. [accessed 2024 Oct 6]. Yob NJ, Jofrry SM, Affandi MMRM, Teh LK, Salleh MZ, Zakaria ZA. 2011. Zingiber zerumbet (L.) Smith: A Review of its Ethnomedicinal, Chemical, and Pharmacological Uses. Evid Based Complement Alternat Med. 2011;2011:543216. https://doi.org/10.1155/2011/54321

Diarrhea was a prevalent condition that affected millions globally each year, representing a significant public health challenge. It often led to acute dehydration, with severe complications particularly impacting vulnerable populations such as the elderly and young children. The condition remained a leading cause of morbidity and mortality, especially in resource- limited settings. Zingiber zerumbet, commonly referred to as shampoo ginger, was a member of the Zingiberaceae family, closely related to Z. officinale (ginger). Traditionally used in medicine, Z. zerumbet was believed to possess antidiarrheal potential due to its rich phytochemical profile, which included anti-inflammatory and antimicrobial compounds. Research on Z. officinale has demonstrated its efficacy in managing diarrhea through mechanisms such as reducing intestinal motility and addressing microbial pathogens. It was hypothesized that Z. zerumbet may exert similar effects, potentially mitigating excessive intestinal secretions and resolving microbial factors contributing to diarrhea. This study investigated the antidiarrheal activity of ethanolic rhizome extract of Zingiber zerumbet using castor oil- induced diarrhea and gastrointestinal motility tests in mice. The extract was administered at 25%, 50%, and 75% of the LD50, with loperamide as the positive control and PNSS with 2% Tween 80 as the negative control. Evaluated parameters included stool frequency, consistency, and intestinal transit using the charcoal meal method. The extract was consistently prepared through ethanol maceration and demonstrated a favorable safety profile, with no signs of toxicity at the administered doses. Pharmacological assessments revealed significant reduction of diarrhea frequency, improvement of stool consistency, and inhibition of gastrointestinal motility compared to controls. These outcomes led to the rejection of the null hypotheses and supported the alternative, confirming a dose-dependent antidiarrheal effect comparable to loperamide. The findings underscore the potential of Z. zerumbet as a safe and effective natural antidiarrheal agent, warranting further exploration for therapeutic use.

Keywords : Zingiber zerumbet, Antidiarrheal, Ethanolic Extract, Rhizome, Castor Oil-Induced Test, Gastrointestinal Motility Test.

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