Welkayt sugar factory has started working in June, 2013, found in western zone of Tigray region, Ethiopia .there are 7,249 permanent workers including 2,494 women and 4, 755 men. It is located at an altitude of 486 meter above sea level .The average annual temperature is 29.90c and there is 16.04mm of annual rain fall. Malaria remains a leading cause of death and ill-health in the developing world. According to the World Health Organization (WHO), there were more than 219 million cases of malaria in 2010, claiming 660,000 lives. At the welkayt sugar factory, malaria is one of the leading causes of morbidity throughout the year. The monthly prevalence of malaria was dramatically increased from 0.24 %( 2) in July/2013, 4.7 %( 38) in August/2013, 7.6 %( 61) in September/2013 to 30.2 %( 241) in October/2013. However, since the coverage of LLITN among the workers of the factory was reportedly nearly 100%, further investigation is needed to understand the unexpectedly high burden of malaria disease. This will be the first special epidemiological investigation study conducted in this area. The target of this study is to identify the incidence of malaria disease for respective parasite species and the risk factors for malaria disease among workers at the Welkayt sugar factory, Tigray region, Ethiopia.we concurred that the place welkayt sugar factory face a significantly higher malaria infection because of the low application of prevention measures ranging from promotion of LLITNs to indoor residual spray (IRS) , environmental vector management and low community awareness towards prevention and control methods of malaria infection.