Stress in a resident's professional life can be triggered by the activities of a specialist doctor's education program who is undergoing a resident without exception anesthesiology. During the COVID-19 pandemic, the incidence of stress in anesthesiology residents at various educational centers in Indonesia is expected to increase as well as in Indonesia, namely at RSUD Soetomo Surabaya. It can be seen that the higher the level of stress experienced by a person, the higher the level of cortisol in the individual's blood. Therefore, these levels were compiled to analyze the relationship of salivary cortisol with stress levels of resident participants in anesthesiology and intensive care at Airlangga University in the era of the Covid-19 pandemic. Methods: This study is an analytical observational study with a cross sectional design which was measured using the Depression, Anxiety, and Stress Scale (DASS)-42 and its relationship with salivary cortisol levels. Results: Through the study, it was found that the salivary cortisol levels of resident participants Anesthesiology and Intensive Carein Medical Faculty of UNAIR before entering the RIK stagnation cycle versus 2 weeks of running RIK stagnation cycle with a median value of 0.315 g/dL versus 0.422 g/dL with (95% CI, 0.143, 0.778) g/dL versus (95% CI, 0.179, 2.802) g/dL with p < 0.05. From the results of the DASS-42 questionnaire obtained 2 subjects (5.7%) mild depression, 1 subject (2.9%) moderate depression, 5 subjects (14.3%) mild anxiety, 1 subject (2.9%) moderate anxiety, 1 subject (2.9%) severe anxiety, 2 subjects (5.7%) mild stress, 5 subjects (14.3%) moderate stress, 1 subject (2.9%) severe stress. Conclusion: Thus, it can be concluded that the stress level in anesthesiology resident participants in the COVID-19 Pandemic era was not stressful or normal because almost 80% of the subjects did not experience stress. There is no significant relationship between salivary cortisol levels and stress levels. Keywords: stress, cortisol, salivary cortisol, COVID-19 pandemic, Anesthesiology residentparticipants.