Authors : V. Rajaiah

Volume/Issue : Volume 11 - 2026, Issue 9 - September

Google Scholar : https://tinyurl.com/3s47hb9s

DOI : https://doi.org/10.38124/ijisrt/26sep950

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Tissue-specific esterase isozyme profiles were investigated in gill, liver, intestine, muscle, brain, and eye tissues of Oreochromis mossambicus and Puntius sarana using electrophoretic zymography and inhibitor-based classification. In O. mossambicus, three esterase zones with relative mobility (Rm) values of 0.96, 0.75, and 0.66 were detected. The Rm 0.96 zone occurred in gill, liver, and intestine and was classified as carboxylesterase (CE). The Rm 0.75 zone was present in all tissues but showed tissue-dependent classification, including CE, esterase resistant to inhibitors (ER), cholinesterase (ChE), and CHsp esterase. The Rm 0.66 zone was present in all tissues except gill and was classified mainly as ER or ChE esterase. In P. sarana, five zones with Rm values of 0.86, 0.75, 0.66, 0.50, and 0.33 were identified. Gill, liver, and intestine showed four esterase zones each, whereas muscle, brain, and eye showed three zones. The Rm 0.50 and 0.33 zones were ubiquitous in P. sarana, while the Rm 0.86 zone was restricted to intestine. ER esterases predominated in gill, liver, intestine, and brain, whereas ChE and CE esterases displayed marked tissue specificity. These findings demonstrate clear interspecific and tissue-dependent heterogeneity in esterase isozyme expression, supporting the value of zymographic inhibitor profiling for biochemical characterization and environmental biomonitoring of freshwater fishes.

Keywords : Esterase Isozymes; Zymography; Carboxylesterase; Cholinesterase; Tissue Specificity; Oreochromis mossambicus; Puntius sarana.

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