Authors :
Sarang Mahajan; Anuradha Khade; Pranaya Pradeep Nampalliwar; Rahul Waghaye
Volume/Issue :
Volume 8 - 2023, Issue 10 - October
Google Scholar :
https://tinyurl.com/yc7csh5s
Scribd :
https://tinyurl.com/yrfenvy6
DOI :
https://doi.org/10.5281/zenodo.10020762
Abstract :
Various micro- and macronutrients play a
vital role in determining crop productivity. This study is
aimed in isolating and identifying Zinc solubilizing
microorganisms from rhizosphere soil. Zinc (Zn) is an
essential trace element for life as it is required for the
structural integrity and catalytic activity of all the classes
of enzymes, and the zinc-binding domains are highly
conserved among species. In plants, these enzymes are
involved in carbohydrate metabolism, auxin metabolism,
and antioxidant activity. The isolation of microorganisms
from soil was implemented by inoculating a 1 mL
dilution over the medium. Zinc oxide was used as an
insoluble zinc source and modified the composition of
Pikovskaya’s agar. The plates were incubated at 37 ̊C for
48 hours, then the bacterial and fungal colonies were
observed, and pure cultures were maintained on a
Nutrient Agar slant and a Sabouraud Dextrose Agar
(SDA) slant, respectively. Microscopic examination was
done to at genus level to study morphology, and growth
rate was observed. Isolates were maintained on
Pikovskaya's agar for further screening of Phosphate
solubilization. Some biochemical tests were performed to
differentiate on the basis of their activities. The bacterial
isolates were identified to be of Pseudomonas spp. while
the fungal isolates were identified to be of Aspergillus
spp., and Penicillium spp. The results showed that the
isolates of Aspergillus spp. were the most abundant
fungus. These results show the enormous potential of
these microorganisms for biofertilizer production.
Keywords :
Zinc Solubilizing Microorganisms, Pseudomonas spp., Aspergillus spp., Penicillium spp.
Various micro- and macronutrients play a
vital role in determining crop productivity. This study is
aimed in isolating and identifying Zinc solubilizing
microorganisms from rhizosphere soil. Zinc (Zn) is an
essential trace element for life as it is required for the
structural integrity and catalytic activity of all the classes
of enzymes, and the zinc-binding domains are highly
conserved among species. In plants, these enzymes are
involved in carbohydrate metabolism, auxin metabolism,
and antioxidant activity. The isolation of microorganisms
from soil was implemented by inoculating a 1 mL
dilution over the medium. Zinc oxide was used as an
insoluble zinc source and modified the composition of
Pikovskaya’s agar. The plates were incubated at 37 ̊C for
48 hours, then the bacterial and fungal colonies were
observed, and pure cultures were maintained on a
Nutrient Agar slant and a Sabouraud Dextrose Agar
(SDA) slant, respectively. Microscopic examination was
done to at genus level to study morphology, and growth
rate was observed. Isolates were maintained on
Pikovskaya's agar for further screening of Phosphate
solubilization. Some biochemical tests were performed to
differentiate on the basis of their activities. The bacterial
isolates were identified to be of Pseudomonas spp. while
the fungal isolates were identified to be of Aspergillus
spp., and Penicillium spp. The results showed that the
isolates of Aspergillus spp. were the most abundant
fungus. These results show the enormous potential of
these microorganisms for biofertilizer production.
Keywords :
Zinc Solubilizing Microorganisms, Pseudomonas spp., Aspergillus spp., Penicillium spp.
