Unveiling the Potential of Zinc Solubilizing Microorganisms for Biofertilizer Application: Isolation and Identification

Authors : Sarang Mahajan; Anuradha Khade; Pranaya Pradeep Nampalliwar; Rahul Waghaye

Volume/Issue : Volume 8 - 2023, Issue 10 - October

Google Scholar : https://tinyurl.com/yc7csh5s

Scribd : https://tinyurl.com/yrfenvy6

DOI : https://doi.org/10.5281/zenodo.10020762

Various micro- and macronutrients play a vital role in determining crop productivity. This study is aimed in isolating and identifying Zinc solubilizing microorganisms from rhizosphere soil. Zinc (Zn) is an essential trace element for life as it is required for the structural integrity and catalytic activity of all the classes of enzymes, and the zinc-binding domains are highly conserved among species. In plants, these enzymes are involved in carbohydrate metabolism, auxin metabolism, and antioxidant activity. The isolation of microorganisms from soil was implemented by inoculating a 1 mL dilution over the medium. Zinc oxide was used as an insoluble zinc source and modified the composition of Pikovskaya’s agar. The plates were incubated at 37 ̊C for 48 hours, then the bacterial and fungal colonies were observed, and pure cultures were maintained on a Nutrient Agar slant and a Sabouraud Dextrose Agar (SDA) slant, respectively. Microscopic examination was done to at genus level to study morphology, and growth rate was observed. Isolates were maintained on Pikovskaya's agar for further screening of Phosphate solubilization. Some biochemical tests were performed to differentiate on the basis of their activities. The bacterial isolates were identified to be of Pseudomonas spp. while the fungal isolates were identified to be of Aspergillus spp., and Penicillium spp. The results showed that the isolates of Aspergillus spp. were the most abundant fungus. These results show the enormous potential of these microorganisms for biofertilizer production.

Keywords : Zinc Solubilizing Microorganisms, Pseudomonas spp., Aspergillus spp., Penicillium spp.


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