Authors : Daniel Dompreh; Collins Ayine Nsor; Adams Latif Mohammed; Philomina Amponsah

Volume/Issue : Volume 11 - 2026, Issue 1 - January

Google Scholar : https://tinyurl.com/jyd5aacj

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DOI : https://doi.org/10.38124/ijisrt/26jan887

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Abstract : Conocarpus erectus is a woody shrub belonging to Combretaceae family. The species provides important ecological and socio-economic benefits to many organisms at coastal areas of Ghana. However, C. erectus is threatened to extinction from wildfires, sand winning, wood collections, farming, building, road construction and illegal mining, leaving just a few fragmented populations that require immediate protection. Little efforts by local communities, Ghana Forestry Commission, and non- governmental organizations to conserve C. erectus through in situ and ex situ methods have been challenging as they relied on general seed collections from fragmented populations without any genetic investigation about these populations. To propose scientific and effective in situ and ex situ conservation of C. erectus in the Central region of Ghana, investigating genetic diversity of the fragmented populations is needed in selecting best genotypes for seed collection. Random Amplified Polymorphic DNA (RAPD) analysis was used. Fifteen primers were screened to ten which were used to produce clearly polymorphic bands with average number of 85.1 per primer. Analysis of Molecular Variance (AMOVA) identified 90% and 10% variance between and within populations respectively. An average number of 10.2 bands per primer was recorded with amplification products ranging between 200 and 2000 bp. PHIst of 0.90 implies significant degree of variability in C. erectus populations, therefore seeds should be collected from all fragmented populations to preserve more of the species’ unique characters for in situ and ex situ conservation of C. erectus.

Keywords : Conservation; Conocarpus; Fragmented Populations; Genotypes; Seed Collection.

References :

1. Allendorf, F. W., Luikart, G., & Aitken, S. N. (2013). Conservation and the genetics of populations. John Wiley and Sons, West Sussex, UK. https://doi.org/10.5860/choice.44-3275
2. Aye, W. N., Wen, Y., Marin, K., Thapa, S., & Tun, A., W. (2019).  Contribution of mangrove forest to the livelihood of local communities in Ayeyarwaddy region, Myanmar. Forests, 10 (414).  https://doi.org/ 10.3390/f10050414
3. Bashir, M., Uzair, M., & Bashir, A. C. (2015). A review of phytochemical and biological studies on Conocarpus erectus (Combretaceae). Pakistan Journal of Pharmaceutical Research, 1(1), 1-8.  https://doi.org/10.22200/pjpr.201511-8
4. Binks, R., M., Byrne, M., McMahon, K., Pitt, G., Murray, K., & Evans, R. D. (2019). Habitat discontinuities form strong barriers to gene flow among mangrove populations, despite the capacity for long-distance dispersal.  Diversity and Distributions, 25(2), 298-309. https://doi.org/10.1111/ddi.12851
5. Bunting, P., Rosenqvist, A., Hilarides, L.,………..& Rebelo,  L. (2022). Global Mangrove Extent Change 1996-2020: Global Mangrove Watch Version 3.0. Remote. Sensing, 14, 3657.
6. Carugati, L., Gatto, B., Rastelli, E., L., ………..& Danovaro,  R. (2018). Impact of mangrove forests degradation on biodiversity and ecosystem functioning. Scientific Reports, 5, 8(1), 13298. https://doi.org/10.1038/s41598-018-31683-0
7. Das, S. C., Das, S., & Tah, J. (2022). Mangrove forests and people’s livelihoods. In mangroves: biodiversity, livelihoods and conservation, Springer Nature, Singapore, 153-173. https://doi.org/10.1007/978-981-19-0519-3_7
8. Das, S., & Crépin, A., S. (2013).  Mangroves can provide protection against wind damage during storms. Estuarine, Coastal and Shelf Science, 134, 98-107.   https://doi.org/10.1016/J.ECSS.2013.09.021
9. Dompreh, D., Swaine, M., & Price, A. (2011). Low genetic diversity and high genetic differentiation among severely fragmented populations of the critically endangered tree Talbotiella gentii (Fabaceae). Southern Forests: A Journal of Forest Science, 73(2), 73–80. https://doi.org/10.2989/20702620.2011.610778
10. Dompreh, D., Price, A., & Swaine, M. D. (2026). High population differentiation of Okpe River Talbotiella gentii at Anum Boso, Ghana, uncovered by RAPD. Southern Forests: A Journal of Forest Science, 1–9. https://doi.org/10.2989/20702620.2025.2528700
11. Excoffier, L., Smouse, P. E., & Quattro, J., M. (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics, 131(2), 479–491. https://doi.org/10.1093/genetics/131.2.479
12. Frankham, R., Ballou, J. D., & Briscoe, D., A. (2002). Introduction to Conservation Genetics. University of Cambridge, Cambridge, UK The Press Syndicate. https://doi.org/10.1017/CBO9780511808999
13. Hadrys, H., Balick, M., & Schierwater, B. (1992). Applications of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology. Molecular Ecology, 1(1), 55-63. https://doi.org/10.1111/j.1365-294X.1992.tb00155.x
14. Hoffmann, A. A., Miller, A., D., & Weeks, A. R. (2020). Genetic mixing for population management: From genetic rescue to provenancing. Evolutionary Applications, 6, 14(3), 634-652.  https://doi.org/10.1111/eva.13154. PMID: 33767740
15. Ivetić, V., & Devetaković, J. (2017). Concerns and Evidence on Genetic Diversity in Planted Forests. Reforesta, 3, 196-207. https://doi.org/10.21750/REFOR.3.15.39
16. Khurm, M., Guo, Y., ………& Wu, Q. (2023). Conocarpus lancifolius (Combretaceae): Pharmacological Effects, LC-ESI-MS/MS Profiling and In Silico Attributes. Metabolites, 13(7), 794. https://doi.org/10.3390/metabo13070794
17. Lowenfeld, R., & Klekowski, E. J. (1992).  Mangrove genetics. I. Mating system and mutation rates of rhizophora mangle in Florida and San Salvador Island, Bahamas. International Journal of Plant Science, 153, 394–399.
18. Mccune, B., & Mefford, M., J. (1999). Multivariate Analysis of Ecological Data. Version 4.25, MjM Software, Gleneden Beach.
19. Namjoyan, F., Farasat, M., Kiabi, S., Ramezani, Z., & Mousavi, H. (2020). Structural and ultra-structural analysis of Conocarpus erectus pollen grains before and after dust storms. Grana, 59(2–3), 226-237. https://doi.org/10.1080/00173134.2019.1689290
20. Nascimento, D., K.,……..& Vieira, J., R. (2016).  Phytochemical screening and acute toxicity of aqueous extract of leaves of Conocarpus erectus Linnaeus in Swiss albino mice. Anais da Academia Brasileira de Ciências, 88 (3), 1431-1437. https://doi.org/10.1590/0001-3765201620150391
21. Nonić, M., & Šijačić-Nikolić, M.  (2021).  Genetic diversity: sources, threats, and conservation. In: Filho, W. L., Azul, A. M., Brandli, L., Salvia, A. L., & Wall, T. (eds.), Life on Land, Springer, 421-435.  https://doi.org/10.1007/978-3-319-95981-8_53
22. Nunoo, F., & Agyekumhene, A. (2022). Mangrove degradation and management practices along the coast of Ghana. Agricultural Science, 13, 1057-1079. https://doi.org/10.4236/as.2022.1310065
23. Olowokudejo, M., & Ozioma, E. (2020). Mangrove and mangrove-associated species richness in selected lagoon and coastal communities in Lagos. Journal of Wetlands and Waste Management, 4(1), 12-22.
24. Oluwajuwon, T., Attafuah, R., Offiah, C., & Krabel, D. (2022). Genetic variation in tropical tree species and plantations: A Review. Open Journal of Forestry, 12, 350-366. https://doi.org/10.4236/ojf.2022.123019
25. Pretzsch, H. (2021). Genetic diversity reduces competition and increases tree growth on a Norway Spruce (Picea abies L KARST.) provenance mixing experiment. Forest Ecology and Management, 497, 119498. https://doi.org/10.1016/j.foreco.2021.119498
26. Raza, S., A., Chaudhary, A., R., Mumtaz, M. W…,……….… & Waheed, A. (2018). Antihyperglycemic effect of Conocarpus erectus leaf extract in alloxan-induced diabeticmice. Pakistan Journal of Pharmaceutical Sciences, 31(2), 637–642.
27. Salgotra, R., K., & Chauhan, B. S. (2023). Genetic Diversity, Conservation, and Utilization of Plant Genetic Resources. Genes (Basel,), 9, 14(1), 174. https://doi.org/10.3390/genes14010174
28. Reynolds, L. K., McGlathery, K. J., Waycott, M. (2012). Genetic diversity enhances restoration success by augmenting ecosystem services. PLoS ONE 7, 1–7.
29. Sandilyan, S., & Kathiresan, K. (2012). Mangrove conservation: a global perspective. Biodiversity Conservation, 21, 3523–3542.  https://doi.org/10.1007/s10531-012-0388-x
30. Santos, D., De-Almeida, V. S., & De Araujo, D., R., C,.,……..& DeSena, K. X. D. F. R. (2018). Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae). Journal of Pharmacy and Pharmacology, 70, 1092–1101. https://doi.org/10. 1111/jphp.12930
31. Tewari, S., Tewari, L., ………& Kaushal, R. (2022). Use of the RAPD marker to determine the genetic diversity of various Dalbergia sissoo Roxb (Shisham) genotypes and their evolutionary relationship in Indian subcontinents. . https://doi.org/10.1007/s42535-021-00334-7
32. Williams, C. E., & Clair, D. A. S. (1993). Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions of Lycopersicon esculentum. Genome, 36(3), 619-630. https://doi.org/10.1139/G93-083
33. Wu, Q., Dong, S., Zhao, Y., ……&. Cheng, J.  (2023). Genetic diversity, population genetic structure and gene flow in the rare and endangered wild plant Cypripedium macranthos revealed by genotyping-by-sequencing. BMC Plant Biology, 23, 254. https://doi.org/10.1139/G93-08310.1186/s12870-023-04212-z