Validation of an Optimized Ultrasound Assisted Green Extraction Method by using Fresh Leaves of Carica papaya


Authors : A F M Nazmus Sadat; Shamima Ahsan; Md. Sohrab ; Nishat Rayhana; Debobrata Sharma; Alamgir Sahriar; Md. Rashedul Islam; Afroza Sultana

Volume/Issue : Volume 6 - 2021, Issue 11 - November

Google Scholar : http://bitly.ws/gu88

Scribd : https://bit.ly/3FCtaxH

Green extraction generally addresses the discovery and design of extraction processes to reduce energy consumption, application of alternative solvents and renewable natural products, and ensure a safe and high quality extract. The proposed “aqueous ultrasound assisted extraction (UAE) from fresh plant material” have been designed based on the green extraction principles. In the present study, fresh leaves of Carica papayawas used for validating the process in comparison with some conventional methods. C. papaya is locally known as pappaiya and widely used as fruits and vegetable in Bangladesh. Traditionally the plant is rich of medicinal components and different parts of the plant are frequently prescribed for several household and folk remedies in different parts of the world. The proposed method was validated based on the extraction yield (%) value, presence of phytochemicals and the antimicrobial sensitivity compared with the reference extraction method. Aqueous UAE form fresh leaves of C. papaya was provide significantly high yield (31.80%) compared to the conventional method indicated the high efficiency of the extraction method. From phytochemical screening tests the presence of Alkaloid, Anthraquinones, Flavonoid, Glycoside, Saponin, Steroid, Tannin, Terpenoid and Vitamin C were observed in the crude extracts which was somewhat even better than the conventional method. The crude extract have observed promising sensitive against Bacillus subtilis and Agrobacterium specieswhich was almost similar to the conventional crude extracts. Based on the present study, the proposed method may be recommended for an effective green extraction procedure suitable for both laboratory and industrial setup.

Keywords : Carica papaya, Bacillus subtilis, Agrobacterium species, Green extraction, Ultrasound Assisted Extraction (UAE), Validation

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