Authors :
A F M Nazmus Sadat; Shamima Ahsan; Md. Sohrab ; Nishat Rayhana; Debobrata Sharma; Alamgir Sahriar; Md. Rashedul Islam; Afroza Sultana
Volume/Issue :
Volume 6 - 2021, Issue 11 - November
Google Scholar :
http://bitly.ws/gu88
Scribd :
https://bit.ly/3FCtaxH
Green extraction generally addresses the
discovery and design of extraction processes to reduce
energy consumption, application of alternative solvents
and renewable natural products, and ensure a safe and
high quality extract. The proposed “aqueous ultrasound
assisted extraction (UAE) from fresh plant material”
have been designed based on the green extraction
principles. In the present study, fresh leaves of Carica
papayawas used for validating the process in comparison
with some conventional methods. C. papaya is locally
known as pappaiya and widely used as fruits and
vegetable in Bangladesh. Traditionally the plant is rich
of medicinal components and different parts of the plant
are frequently prescribed for several household and folk
remedies in different parts of the world. The proposed
method was validated based on the extraction yield (%)
value, presence of phytochemicals and the antimicrobial
sensitivity compared with the reference extraction
method. Aqueous UAE form fresh leaves of C. papaya
was provide significantly high yield (31.80%) compared
to the conventional method indicated the high efficiency
of the extraction method. From phytochemical screening
tests the presence of Alkaloid, Anthraquinones,
Flavonoid, Glycoside, Saponin, Steroid, Tannin,
Terpenoid and Vitamin C were observed in the crude
extracts which was somewhat even better than the
conventional method. The crude extract have observed
promising sensitive against Bacillus subtilis and
Agrobacterium specieswhich was almost similar to the
conventional crude extracts. Based on the present study,
the proposed method may be recommended for an
effective green extraction procedure suitable for both
laboratory and industrial setup.
Keywords :
Carica papaya, Bacillus subtilis, Agrobacterium species, Green extraction, Ultrasound Assisted Extraction (UAE), Validation