Authors : Astija; Samsurizal M. Suleman; Mursito S. Bialangi; Vita Indri Febriani; Nurul Afiat
Volume/Issue : Volume 8 - 2023, Issue 8 - August
Google Scholar : https://bit.ly/3TmGbDi
Scribd : https://tinyurl.com/5hd9fb7y
DOI : https://doi.org/10.5281/zenodo.8303945
Tadulako University and the Polytechnic are
illustrative instances of higher education establishments
in Palu City that are consistently enhancing the
capabilities and expertise of their faculty members,
equipping them with the necessary qualifications to
confront the ever-evolving challenges posed by
advancements in science and technology, particularly in
the domain of laboratory research. One essential skill he
must acquire is proficiency in employing
biophotomicrograph methods. The implementation of
this technique is crucial for study programs that
integrate advocacy and academic education in the
laboratory domain. Its extensive and frequent utilization
by students and instructors is highly beneficial for their
engagement in practical and analytical activities, as well
as research endeavors in the areas of health,
microtechnics, micromaterials, and micropreparation.
Based on the current observations and debates, it
appears that these research institutes do not
acknowledge the validity or significance of
biophotomicrographs. Hence, the implementation of this
technical training activity is deemed highly essential. The
aforementioned activity was conducted over a span of
two consecutive days, specifically from August 9th to
August 10th in the year 2023. The activity encompassed
a two-day session comprising theoretical instruction on
the fundamental principles of biophotomicrograph
methods, followed by a practical session involving the
measurement, capture, and analysis of microscopic
pictures. In practical endeavors, photomicrograph
modification techniques are utilized to facilitate various
procedures. Photomicrographs are generated by affixing
a smartphone to the eyepiece while manually grasping
the smartphone. The calibration procedure was
thereafter conducted with a micrometer measuring 10
μm in size. Subsequently, proceed with the installation of
the ocular micrometer within the eyepiece. The scale that
becomes visible is thereafter aligned with both the ocular
micrometer scale and the objective micrometer scale.
The outcomes of the ocular micrometer calibration are
afterwards employed for the direct or indirect
measurement of microscopic entities. This is achieved by
initially capturing images using a smartphone and
subsequently quantifying them with the assistance of the
ImageJ software. Consequently, the aforementioned
endeavor successfully accomplished its objective of
enhancing the comprehension, attitudes, and
competencies of educational personnel in relation to the
biophotomicrograph technology. Additionally, it yielded
a refined version of a photomicrograph prototype that
had the capability to measure micro-objects in
accordance with global benchmarks.
Keywords : Biophotomicrograph, Imagej, Micrometer, Microscope