Biophotomicrograph, an Alternative Technique for Measuring Micro-Sized Biological Objects

Authors : Astija; Samsurizal M. Suleman; Mursito S. Bialangi; Vita Indri Febriani; Nurul Afiat

Volume/Issue : Volume 8 - 2023, Issue 8 - August

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Tadulako University and the Polytechnic are illustrative instances of higher education establishments in Palu City that are consistently enhancing the capabilities and expertise of their faculty members, equipping them with the necessary qualifications to confront the ever-evolving challenges posed by advancements in science and technology, particularly in the domain of laboratory research. One essential skill he must acquire is proficiency in employing biophotomicrograph methods. The implementation of this technique is crucial for study programs that integrate advocacy and academic education in the laboratory domain. Its extensive and frequent utilization by students and instructors is highly beneficial for their engagement in practical and analytical activities, as well as research endeavors in the areas of health, microtechnics, micromaterials, and micropreparation. Based on the current observations and debates, it appears that these research institutes do not acknowledge the validity or significance of biophotomicrographs. Hence, the implementation of this technical training activity is deemed highly essential. The aforementioned activity was conducted over a span of two consecutive days, specifically from August 9th to August 10th in the year 2023. The activity encompassed a two-day session comprising theoretical instruction on the fundamental principles of biophotomicrograph methods, followed by a practical session involving the measurement, capture, and analysis of microscopic pictures. In practical endeavors, photomicrograph modification techniques are utilized to facilitate various procedures. Photomicrographs are generated by affixing a smartphone to the eyepiece while manually grasping the smartphone. The calibration procedure was thereafter conducted with a micrometer measuring 10 μm in size. Subsequently, proceed with the installation of the ocular micrometer within the eyepiece. The scale that becomes visible is thereafter aligned with both the ocular micrometer scale and the objective micrometer scale. The outcomes of the ocular micrometer calibration are afterwards employed for the direct or indirect measurement of microscopic entities. This is achieved by initially capturing images using a smartphone and subsequently quantifying them with the assistance of the ImageJ software. Consequently, the aforementioned endeavor successfully accomplished its objective of enhancing the comprehension, attitudes, and competencies of educational personnel in relation to the biophotomicrograph technology. Additionally, it yielded a refined version of a photomicrograph prototype that had the capability to measure micro-objects in accordance with global benchmarks.

Keywords : Biophotomicrograph, Imagej, Micrometer, Microscope


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31 - December - 2023

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