Bioreactors, also called fermenters, are used in the production of vaccine antigens. However, various parameters (temperature, pressure, agitation, pH, oxygen concentration) must be carefully controlled to ensure the right conditions inside the bioreactor. Indeed, the good control of these parameters ensures a successful production and a high yield. In this work, an automatic real-time pH monitoring and adjustment system is developed. The system is composed of a device made with various materials and specific computer programs. The device is needed to detect the pH value in the bioreactors while the programs are used to control and parameterize the device. The device is called "pH control kit" and consists of a pH sensor, a processing unit, a switching hardware and a transmission hardware. The device is controlled by software and two types of programs called "pH control", "Arduino" program and "Java" program respectively. The "pH control kit" works for different types of bioreactors, however, the control programs can be customized for different types of antigens. The results show that it is possible to automatically detect and adjust the pH value in a culture medium in real time. Thus, the device and control programs can be a valuable tool for optimizing bioreactor-based antigen production processes.

Keywords : bioreactors, pH, automatic, pH control kit, control programs, antigens.