Phytochemicals, the natural biochemical substances produced by plants possess a range of medicinal values. These phytochemicals get into our system through food and bring different physiological benefits. However, many of these phytochemicals lack essential physicochemical properties that can provide them effective drug-likeness properties. Tylophora indica alkaloids are known for their therapeutic values especially in the cancer treatment domain. However, the physiochemical properties of these molecules limit their bioavailability greatly. This current research aimed at improving the bioavailability of Tylophora indicaalkaloids through the nanovector system, niosomes. Tylophora indica alkaloids were extracted, purified, screened and quantified. Niosomal constructs of the alkaloids were made, characterized through scanning electron microscope and ex- vivo studies were carried out using goat intestine. Results shows that niosomal formulation can increase bioavailability of Tylophora indica alkaloidstwo folds compared to the native alkaloid extract.