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Extraction and Biosynthesis of Silver Nanoparticles from Allium sativum Essential Oil


Authors : Dax Dubey; Monika Yadav; Samakshi Verma

Volume/Issue : Volume 11 - 2026, Issue 5 - May


Google Scholar : https://tinyurl.com/2e3hwvj7

Scribd : https://tinyurl.com/yuxsexeu

DOI : https://doi.org/10.38124/ijisrt/26May388

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : Garlic was used as a natural reducing and stabilizing agent for the creation of nanoparticles since it is a rich source of organosulfur compounds. 29.58% of the essential oil was extracted using Soxhlet extraction with ethanol as a solvent, demonstrating effective extraction under ideal circumstances. Under carefully regulated pH and temperature settings, diluted garlic oil was reacted with an aqueous silver nitrate (AgNO₃) solution to produce AgNPs. The creation of spherical silver nanoparticles was confirmed by characterization using UV–visible spectroscopy, which showed a clear absorption peak at 420 nm. The limited peak range suggested that the nanoparticles were modestly distributed and relatively stable. Furthermore, Gram staining and microbial isolation verified that soil samples included Gram-positive bacteria, which were utilized for additional antibacterial assessment. The study shows that the manufacture of silver nanoparticles using garlic essential oil is an efficient, economical and environmentally friendly method. This study emphasizes the importance of green nanotechnology derived from plants as a sustainable substitute for traditional chemical synthesis techniques.

Keywords : Garlic Essential oil, Allium sativum, Silver Nanoparticles, Green Synthesis, UV–Vis Spectroscopy, Antimicrobial Activity, Surface plasmon Resonance, etc

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Garlic was used as a natural reducing and stabilizing agent for the creation of nanoparticles since it is a rich source of organosulfur compounds. 29.58% of the essential oil was extracted using Soxhlet extraction with ethanol as a solvent, demonstrating effective extraction under ideal circumstances. Under carefully regulated pH and temperature settings, diluted garlic oil was reacted with an aqueous silver nitrate (AgNO₃) solution to produce AgNPs. The creation of spherical silver nanoparticles was confirmed by characterization using UV–visible spectroscopy, which showed a clear absorption peak at 420 nm. The limited peak range suggested that the nanoparticles were modestly distributed and relatively stable. Furthermore, Gram staining and microbial isolation verified that soil samples included Gram-positive bacteria, which were utilized for additional antibacterial assessment. The study shows that the manufacture of silver nanoparticles using garlic essential oil is an efficient, economical and environmentally friendly method. This study emphasizes the importance of green nanotechnology derived from plants as a sustainable substitute for traditional chemical synthesis techniques.

Keywords : Garlic Essential oil, Allium sativum, Silver Nanoparticles, Green Synthesis, UV–Vis Spectroscopy, Antimicrobial Activity, Surface plasmon Resonance, etc

Paper Submission Last Date
31 - May - 2026

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