Study and Isolation of Skin Microflora and their Effects on Human Health


Authors : Dr Madhulika Singh, Priya Rathore, Shuchi Gupta.

Volume/Issue : Volume 3 - 2018, Issue 9 - September

Abstract : Staphylococcus species are Gram positive, cocci shaped bacteria found especially in the wounds of a person. Skin micro flora is the major source of this species and present in the upper epidermis of it. Skin samples were collected to isolate the Staphylococcus bacteria and studied their resistance towards antibiotics. In this thesis, samples of different communities were taken by sterilized swap sticks and inoculate. Mannitol Salt Agar Media specialized for Staphylococcus Species was used to culture the incubated broth tubes to study the morphology. Biochemical test and Antibiotic Sensitivity Tests were performed to analyze the enzyme activity and Sensitivity Resistance towards antibiotics, respectively. This study revealed the two species of Staphylococcus- aureus and epidermidis and their morphology and biochemical characteristics along with resistance towards some antibiotics.

Staphylococcus species are Gram positive, cocci shaped bacteria found especially in the wounds of a person. Skin micro flora is the major source of this species and present in the upper epidermis of it. Skin samples were collected to isolate the Staphylococcus bacteria and studied their resistance towards antibiotics. In this thesis, samples of different communities were taken by sterilized swap sticks and inoculate. Mannitol Salt Agar Media specialized for Staphylococcus Species was used to culture the incubated broth tubes to study the morphology. Biochemical test and Antibiotic Sensitivity Tests were performed to analyze the enzyme activity and Sensitivity Resistance towards antibiotics, respectively. This study revealed the two species of Staphylococcus- aureus and epidermidis and their morphology and biochemical characteristics along with resistance towards some antibiotics.

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